Effects of different cytokines on immune responses of rainbow trout in a virus DNA vaccination model
文献类型：期刊论文
| 作者 | Cao, Yongsheng1,2; Zhang, Qiya2; Xu, Liming1; Li, Shaowu1; Wang, Di1; Zhao, Jingzhuang1; Liu, Hongbai1; Feng, Jian3; Lu, Tongyan1 |
| 刊名 | ONCOTARGET
 |
| 出版日期 | 2017-12-22 |
| 卷号 | 8期号:68页码:112222-112235 |
| 关键词 | rainbow trout cytokines adjuvant DNA vaccine protection Immune response Immunology |
| ISSN号 | 1949-2553 |
| DOI | 10.18632/oncotarget.23095 |
| 英文摘要 | Seven rainbow trout cytokine genes (interleukin (IL)-2, IL-8, IL-15, IL-17, IL-1 beta, intracellular interferon (iIFN) 1a, and IFN-gamma 2) were evaluated for their adjuvant effects on a DNA vaccine, called pG, containing the glycoprotein gene of infectious hematopoietic necrosis virus (IHNV). Distinct DNA constructs in expression plasmid pcDNA3.1 encoding a cytokine gene were generated. Immunofluorescence assays in rainbow trout gonadal cells demonstrated successful protein expression from all these constructs. Subsequently, fish were immunized with pG alone or together with a cytokine expression plasmid. Results showed that each cytokine plasmids at an appropriate dose showed notable effects on immune gene expression. IL-17 and IFN-gamma 2 can enhance early specific IgM response. All cytokines, except IL-8, can benefit initial neutralizing antibody (NAb) titers. At 35 days post immunization (dpi), NAb titers of fish immunized with pG and IL-2, iIFN1a, or IFN-gamma 2 plasmids remained at high levels (1: 160). NAb titers of fish immunized with pG alone decreased to 1: 40. IL-8 or IL-1 beta can enhance antigen-specific proliferative T-cell responses at 14 dpi. At 28 dpi, coinjection of pG with IL-2, IL-8, IL-15, or IL-17 plasmids induced considerably stronger lymphocyte proliferation than that with injection of pG alone. All cytokine plasmids delivered with pG plasmid enhanced protection of trout against IHNV-mediated mortality. These results indicate that the type and dose of trout cytokine genes injected into fish affect quality of immune response to DNA vaccination. |
| 资助项目 | Central-level Nonprofit Scientific Research Institutes Special Funds[HSY201702M] ; China Postdoctoral Science Foundation[2016M602395] ; State Key Laboratory of Freshwater Ecology and Biotechnology[2017FB03] |
| WOS研究方向 | Oncology ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000419569800007 |
| 出版者 | IMPACT JOURNALS LLC |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/4532]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Zhang, Qiya; Lu, Tongyan |
| 作者单位 | 1.Chinese Acad Fishery Sci, Heilongjiang River Fishery Res Inst, Harbin, Heilongjiang, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei, Peoples R China 3.Benxi Agrimarine Co Ltd, Benxi, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cao, Yongsheng,Zhang, Qiya,Xu, Liming,et al. Effects of different cytokines on immune responses of rainbow trout in a virus DNA vaccination model[J]. ONCOTARGET,2017,8(68):112222-112235. |
| APA | Cao, Yongsheng.,Zhang, Qiya.,Xu, Liming.,Li, Shaowu.,Wang, Di.,...&Lu, Tongyan.(2017).Effects of different cytokines on immune responses of rainbow trout in a virus DNA vaccination model.ONCOTARGET,8(68),112222-112235. |
| MLA | Cao, Yongsheng,et al."Effects of different cytokines on immune responses of rainbow trout in a virus DNA vaccination model".ONCOTARGET 8.68(2017):112222-112235. |
入库方式： OAI收割
来源：武汉植物园
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