Comparative Transcriptome Analysis Identifies Putative Genes Involved in Steroid Biosynthesis in Euphorbia tirucalli
文献类型：期刊论文
| 作者 | Li, Changfu1; Mosongo, Isidore1,2; Liang, Qin1,2; Liu, Mengdi1,2; Wang, Xin1; Qiao, Weibo1,2 |
| 刊名 | GENES
 |
| 出版日期 | 2018 |
| 卷号 | 9期号:1页码:14 |
| 关键词 | Euphorbia tirucalli oxidosqualene cyclase transcriptome steroid |
| ISSN号 | 2073-4425 |
| DOI | 10.3390/genes9010038 |
| 英文摘要 | Phytochemical analysis of different Euphorbia tirucalli tissues revealed a contrasting tissue-specificity for the biosynthesis of euphol and beta-sitosterol, which represent the two pharmaceutically active steroids in E. tirucalli. To uncover the molecular mechanism underlying this tissue-specificity for phytochemicals, a comprehensive E. tirucalli transcriptome derived from its root, stem, leaf and latex was constructed, and a total of 91,619 unigenes were generated with 51.08% being successfully annotated against the non-redundant (Nr) protein database. A comparison of the transcriptome from different tissues discovered members of unigenes in the upstream steps of sterol backbone biosynthesis leading to this tissue-specific sterol biosynthesis. Among them, the putative oxidosqualene cyclase (OSC) encoding genes involved in euphol synthesis were notably identified, and their expressions were significantly up-regulated in the latex. In addition, genome-wide differentially expressed genes (DEGs) in the different E. tirucalli tissues were identified. The cluster analysis of those DEGs showed a unique expression pattern in the latex compared with other tissues. The DEGs identified in this study would enrich the insights of sterol biosynthesis and the regulation mechanism of this latex-specificity. |
| 资助项目 | Sino-Africa joint research project[SAJC20160233] |
| WOS研究方向 | Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000424116500035 |
| 出版者 | MDPI AG |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/4734]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Wang, Xin |
| 作者单位 | 1.Chinese Acad Sci, CAS Key Lab Plant Germplasm Enhancement & Special, Wuhan Bot Garden, Wuhan 430074, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Changfu,Mosongo, Isidore,Liang, Qin,et al. Comparative Transcriptome Analysis Identifies Putative Genes Involved in Steroid Biosynthesis in Euphorbia tirucalli[J]. GENES,2018,9(1):14. |
| APA | Li, Changfu,Mosongo, Isidore,Liang, Qin,Liu, Mengdi,Wang, Xin,&Qiao, Weibo.(2018).Comparative Transcriptome Analysis Identifies Putative Genes Involved in Steroid Biosynthesis in Euphorbia tirucalli.GENES,9(1),14. |
| MLA | Li, Changfu,et al."Comparative Transcriptome Analysis Identifies Putative Genes Involved in Steroid Biosynthesis in Euphorbia tirucalli".GENES 9.1(2018):14. |
入库方式： OAI收割
来源：武汉植物园
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。