Disaccharide combinations and the expression of enolase3 and plasma membrane Ca2+ ATPase isoform in sturgeon sperm cryopreservation
文献类型：期刊论文
| 作者 | Xi, M. D.1,2,3,4; Li, P.1,4,5; Du, H.1,4; Qiao, X. M.1,4; Liu, Z. G.1,4; Wei, Q. W.1,2,3,4 |
| 刊名 | REPRODUCTION IN DOMESTIC ANIMALS
 |
| 出版日期 | 2018-04-01 |
| 卷号 | 53期号:2页码:472-483 |
| 关键词 | cryopreservation disaccharides enolase3 plasma membrane Ca2+-ATPase isoform sperm |
| ISSN号 | 0936-6768 |
| DOI | 10.1111/rda.13134 |
| 英文摘要 | ContentsAcipenser sinensis and Acipenser dabryanus are critically endangered species, so germplasm conservation via cryopreservation of sperm is necessary. Disaccharides can act as membrane-impermeable cryoprotectants, and enolase3 (ENO3) and plasma membrane Ca2+ ATPase isoform (PMCA2) are proteins associated with sperm quality. We considered seven characteristics of sperm quality in cultured brood stock from A.sinensis and A.dabryanus. We tested use of sucrose or trehalose alone and in combination at different concentrations for cryopreservation of A.dabryanus sperm. A low concentration of sucrose plus trehalose (S15T15) was optimal. Mixing of the extender with sucrose, lactose, or trehalose alone or with pairwise mixtures revealed that a mixture of lactose and trehalose (L15T15) gave the best results for both A.sinensis and A.dabryanus. Enolase3 and PMCA2 expression levels were measured in cryopreserved A.sinensis sperm via Western blotting. Relative ENO3 and PMCA2 expression levels were examined, and the relationship between disaccharide composition, sperm quality and protein expression was explored in A.sinensis. The results showed that relative ENO3 and PMCA2 expression levels were the highest at L15T15 in cryopreserved A.sinensis sperm. There were significant positive correlations between ENO3 expression and percentage membrane integrity, and between PMCA2 expression and sperm motility parameters (percentage of motile sperm, curvilinear velocity, straight-line velocity and average path velocity; p<.05) in cryopreserved A.sinensis sperm. Our results indicate the optimal disaccharide combination and concentrations for cryopreservation of A.sinensis and A.dabryanus sperm and suggest that ENO3 and PMCA2 expression levels could serve as a valuable indicator of sperm quality in A.sinensis. |
| 资助项目 | National Natural Science Foundation of China[31402301] ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences[2015B02YQ01] ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences[2016GH04] ; National Program on Key Basic Research Project[2015CB150702] ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China[201203086] ; Ministry of Education, Youth and Sports of the Czech Republic-project CENAKVA[CZ.1.05/2.1.00/01.0024] ; Ministry of Education, Youth and Sports of the Czech Republic-project CENAKVA II[LO1205] ; Czech Science Foundation[16-02407Y] |
| WOS研究方向 | Agriculture ; Reproductive Biology ; Veterinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000427140800025 |
| 出版者 | WILEY |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/4786]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Wei, Q. W. |
| 作者单位 | 1.Chinese Acad Fishery Sci, Sino Czech Joint Lab Fish Conservat & Biotechnol, Yangtze River Fisheries Res Inst, Wuhan, Hubei, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Hubei, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Key Lab Freshwater Biodivers Conservat, Minist Agr China, Wuhan, Hubei, Peoples R China 5.Univ South Bohemia Ceske Budejovice, Fac Fisheries & Protect Waters, South Bohemian Res Ctr Aquaculture & Biodivers Hy, Res Inst Fish Culture & Hydrobiol, Vodany, Czech Republic |
| 推荐引用方式 GB/T 7714 | Xi, M. D.,Li, P.,Du, H.,et al. Disaccharide combinations and the expression of enolase3 and plasma membrane Ca2+ ATPase isoform in sturgeon sperm cryopreservation[J]. REPRODUCTION IN DOMESTIC ANIMALS,2018,53(2):472-483. |
| APA | Xi, M. D.,Li, P.,Du, H.,Qiao, X. M.,Liu, Z. G.,&Wei, Q. W..(2018).Disaccharide combinations and the expression of enolase3 and plasma membrane Ca2+ ATPase isoform in sturgeon sperm cryopreservation.REPRODUCTION IN DOMESTIC ANIMALS,53(2),472-483. |
| MLA | Xi, M. D.,et al."Disaccharide combinations and the expression of enolase3 and plasma membrane Ca2+ ATPase isoform in sturgeon sperm cryopreservation".REPRODUCTION IN DOMESTIC ANIMALS 53.2(2018):472-483. |
入库方式： OAI收割
来源：武汉植物园
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