Chromosome Nomenclature and Cytological Characterization of Sacred Lotus
文献类型：期刊论文
| 作者 | Yu, Qingyi4; Meng, Zhuang1; Hu, Xiaoxu1; Zhang, Zhiliang1; Li, Zhanjie1; Lin, Qingfang1; Yang, Mei3; Yang, Pingfang3; Ming, Ray1,5; Wang, Kai1,2 |
| 刊名 | CYTOGENETIC AND GENOME RESEARCH
 |
| 出版日期 | 2017 |
| 卷号 | 153期号:4页码:223-231 |
| 关键词 | Chromosome nomenclature Cytological marker Fluorescence in situ hybridization Megascaffold Sacred lotus |
| ISSN号 | 1424-8581 |
| DOI | 10.1159/000486777 |
| 英文摘要 | Sacred lotus is a basal eudicot plant that has been cultivated in Asia for over 7,000 years for its agricultural, ornamental, religious, and medicinal importance. A notable characteristic of lotus is the seed longevity. Extensive endeavors have been devoted to dissect its genome assembly, including the variety China Antique, which germinated from a 1,300-year-old seed. Here, cytogenetic markers representing the 10 largest megascaffolds, which constitute approximately 70% of the lotus genome assembly, were developed. These 10 megascaffolds were then anchored to the corresponding lotus chromosomes by fluorescence in situ hybridization using these cytogenetic markers, and a set of chromosome-specific cytogenetic markers that could unambiguously identify each of the 8 chromosomes was generated. Karyotyping was conducted, and a nomenclature based on chromosomal length was established for the 8 chromosomes of China Antique. Comparative karyotyping revealed relatively conserved chromosomal structures between China Antique and 3 modern cultivars. Interestingly, significant variations in the copy number of 45S rDNA were detected between China Antique and modern cultivars. Our results provide a comprehensive view on the chromosomal structure of sacred lotus and will facilitate further studies and the genome assembly of lotus. (c) 2018 S. Karger AG, Basel |
| 资助项目 | National Natural Science Foundation of China[31471170] ; National Natural Science Foundation of China[31771862] ; National Natural Science Foundation of China[31628013] ; National Engineering Research Center of Sugarcane Open Fund |
| WOS研究方向 | Cell Biology ; Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000428815300007 |
| 出版者 | KARGER |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/5031]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Wang, Kai |
| 作者单位 | 1.Fujian Agr & Forestry Univ, Ctr Genom & Biotechnol, Fujian Prov Key Lab Haixia Appl Plant Syst Biol,M, Key Lab Genet Breeding & Multiple Utilizat Crops, Fuzhou 350002, Fujian, Peoples R China 2.Fujian Agr & Forestry Univ, Natl Engn Res Ctr Sugarcane, Fuzhou, Fujian, Peoples R China 3.Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Special Agr, Wuhan Bot Garden, Wuhan, Hubei, Peoples R China 4.Texas A&M Univ Syst, Dept Plant Pathol & Microbiol, Texas A&M AgriLife Res, Dallas, TX USA 5.Univ Illinois, Dept Plant Biol, Urbana, IL USA |
| 推荐引用方式 GB/T 7714 | Yu, Qingyi,Meng, Zhuang,Hu, Xiaoxu,et al. Chromosome Nomenclature and Cytological Characterization of Sacred Lotus[J]. CYTOGENETIC AND GENOME RESEARCH,2017,153(4):223-231. |
| APA | Yu, Qingyi.,Meng, Zhuang.,Hu, Xiaoxu.,Zhang, Zhiliang.,Li, Zhanjie.,...&Wang, Kai.(2017).Chromosome Nomenclature and Cytological Characterization of Sacred Lotus.CYTOGENETIC AND GENOME RESEARCH,153(4),223-231. |
| MLA | Yu, Qingyi,et al."Chromosome Nomenclature and Cytological Characterization of Sacred Lotus".CYTOGENETIC AND GENOME RESEARCH 153.4(2017):223-231. |
入库方式： OAI收割
来源：武汉植物园
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