Enhanced Replication of Virulent Newcastle Disease Virus in Chicken Macrophages Is due to Polarized Activation of Cells by Inhibition of TLR7
文献类型：期刊论文
| 作者 | Bi, Yuhai7; Zhang, Pingze1; Ding, Zhuang1; Liu, Xinxin2; Chen, Yanyu1; Li, Junjiao1; Tao, Zhi1; Fei, Yidong1; Xue, Cong1; Qian, Jing1 |
| 刊名 | FRONTIERS IN IMMUNOLOGY
 |
| 出版日期 | 2018-04-04 |
| 卷号 | 9页码:13 |
| ISSN号 | 1664-3224 |
| 关键词 | chicken macrophages newcastle disease virus toll-like receptor 7 macrophage polarized activation virus growth immune response |
| DOI | 10.3389/fimmu.2018.00366 |
| 英文摘要 | Newcastle disease (ND), caused by infections with virulent strains of Newcastle disease virus (NDV), is one of the most important infectious disease affecting wild, peridomestic, and domestic birds worldwide. Vaccines constructed from live, low-virulence (lentogenic) viruses are the most accepted prevention and control strategies for combating ND in poultry across the globe. Avian macrophages are one of the first cell lines of defense against microbial infection, responding to signals in the microenvironment. Although macrophages are considered to be one of the main target cells for NDV infection in vivo, very little is known about the ability of NDV to infect chicken macrophages, and virulence mechanisms of NDV as well as the polarized activation patterns of macrophages and correlation with viral infection and replication. In the present study, a cell culture model (chicken bone marrow macrophage cell line HD11) and three different virulence and genotypes of NDV (including class II virulent NA-1, class II lentogenic LaSota, and class I lentogenic F55) were used to solve the above underlying questions. Our data indicated that all three NDV strains had similar replication rates during the early stages of infection. Virulent NDV titers were shown to increase compared to the other lentogenic strains, and this growth was associated with a strong upregulation of both pro-inflammatory M1-like markers/cytokines and anti-inflammatory M2-like markers/cytokines in chicken macrophages. Virulent NDV was found to block toll-like receptor (TLR) 7 expression, inducing higher expression of type I interferons in chicken macrophages at the late stage of viral infection. Only virulent NDV replication can be inhibited by pretreatment with TLR7 ligand. Overall, this study demonstrated that virulent NDV activates a M1-/M2-like mixed polarized activation of chicken macrophages by inhibition of TLR7, resulting in enhanced replication compared to lentogenic viruses. |
| 资助项目 | National Science Foundation of China[31402195] ; National Science Foundation of China[31472195] ; Key Project of Chinese National Programs for Research and Development[2017YFD0500800] ; Natural Science Foundation of Jilin Province[20160414029GH] ; Chinese Special Fund for Agri-scientific Research in the public interest[201303033] |
| WOS研究方向 | Immunology |
| 语种 | 英语 |
| 出版者 | FRONTIERS MEDIA SA |
| WOS记录号 | WOS:000429169400001 |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/5037]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Yin, Renfu |
| 作者单位 | 1.Jilin Univ, Dept Vet Prevent Med, Coll Vet Med, Changchun, Jilin, Peoples R China 2.Jilin Univ, Coll Food Sci & Engn, Changchun, Jilin, Peoples R China 3.Inner Mongolia Univ Nationalities, Coll Anim Sci & Technol, Tongliao, Peoples R China 4.Henan Acad Agr Sci, Lab Anim Immunol, Zhengzhou, Henan, Peoples R China 5.Helmholtz Zentrum Muenchen, Comprehens Pneumol Ctr, iLBD, Munich, Germany 6.Chinese Acad Sci, CAS Key Lab Special Pathogens & Biosafety, Wuhan Inst Virol, Wuhan, Hubei, Peoples R China 7.Chinese Acad Sci, CAS Key Lab Pathogen Microbiol & Immunol, Inst Microbiol, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bi, Yuhai,Zhang, Pingze,Ding, Zhuang,et al. Enhanced Replication of Virulent Newcastle Disease Virus in Chicken Macrophages Is due to Polarized Activation of Cells by Inhibition of TLR7[J]. FRONTIERS IN IMMUNOLOGY,2018,9:13. |
| APA | Bi, Yuhai.,Zhang, Pingze.,Ding, Zhuang.,Liu, Xinxin.,Chen, Yanyu.,...&Yin, Renfu.(2018).Enhanced Replication of Virulent Newcastle Disease Virus in Chicken Macrophages Is due to Polarized Activation of Cells by Inhibition of TLR7.FRONTIERS IN IMMUNOLOGY,9,13. |
| MLA | Bi, Yuhai,et al."Enhanced Replication of Virulent Newcastle Disease Virus in Chicken Macrophages Is due to Polarized Activation of Cells by Inhibition of TLR7".FRONTIERS IN IMMUNOLOGY 9(2018):13. |
入库方式： OAI收割
来源：武汉植物园
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