Screening for anti-proliferative and anti-inflammatory components from Rhamnus davurica Pall. using bio-affinity ultrafiltration with multiple drug targets
文献类型：期刊论文
| 作者 | Guo, Mingquan1,2; Wu, Jianlin3; Chen, Guilin1,2; Li, Na3 |
| 刊名 | ANALYTICAL AND BIOANALYTICAL CHEMISTRY
 |
| 出版日期 | 2018-06-01 |
| 卷号 | 410期号:15页码:3587-3595 |
| ISSN号 | 1618-2642 |
| 关键词 | Rhamnus davurica Bio-affinity ultrafiltration Anti-inflammation Anti-proliferative |
| DOI | 10.1007/s00216-018-0953-6 |
| 英文摘要 | Rhamnus davurica Pall. (R. davurica) has been used as a traditional medicine for many years in China and abroad and shown a wide spectrum of biological activities. Previously, we reported the phytochemical fingerprinting profile of R. davurica, its distinct anti-proliferative activities against HT-29 and SGC-7901 cell lines, and the topoisomerase I (Top I) ligands based on bio-affinity ultrafiltration and HPLC-MS (UF-HPLC-MS). Nevertheless, among the 32 peaks detected in the fingerprinting profile, the common bioactive constituents responsible for the anti-inflammatory and anti-proliferative activities in the extracts remain elusive. To further explore the specific responsible components for their diversified activities and their potential action targets/mechanisms, the method based on bio-affinity UF-HPLC-MS using therapeutic targets like Top I and cyclooxygenase 2 (COX-2) was established to rapidly screen and identify the ligands binding to these known target enzymes. As a result, 12 components were revealed as potential Top I ligands along with 11 components as potential COX-2 ligands, where several components were revealed to possess both activities. Further validations of these bioactive components have also been conducted and confirmed their highlighted activities. This integrated method of UF-HPLC-MS exhibits high efficiency in rapidly screening for multi-target bioactive components responsible for multiple pharmacological effects from the complex natural products and could be very useful to explain the complex action mechanisms of herb medicines in a complex multi-component and multi-target mode at the molecular level. |
| 资助项目 | Natural Science Foundation of China[81673580] ; Chinese Academy of Sciences[29Y429291a0129] ; Major Project for Special Technology Innovation of Hubei Province-Sino-foreign Cooperation Project[2017AHB054] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| 出版者 | SPRINGER HEIDELBERG |
| WOS记录号 | WOS:000431928900015 |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/5212]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Guo, Mingquan |
| 作者单位 | 1.Chinese Acad Sci, Sino Africa Joint Res Ctr, Wuhan 430074, Hubei, Peoples R China 2.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Hubei, Peoples R China 3.Macau Univ Sci & Technol, State Key Lab Qual Res Chinese Med, Ave Wai Long, Taipa, Macao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Mingquan,Wu, Jianlin,Chen, Guilin,et al. Screening for anti-proliferative and anti-inflammatory components from Rhamnus davurica Pall. using bio-affinity ultrafiltration with multiple drug targets[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2018,410(15):3587-3595. |
| APA | Guo, Mingquan,Wu, Jianlin,Chen, Guilin,&Li, Na.(2018).Screening for anti-proliferative and anti-inflammatory components from Rhamnus davurica Pall. using bio-affinity ultrafiltration with multiple drug targets.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,410(15),3587-3595. |
| MLA | Guo, Mingquan,et al."Screening for anti-proliferative and anti-inflammatory components from Rhamnus davurica Pall. using bio-affinity ultrafiltration with multiple drug targets".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 410.15(2018):3587-3595. |
入库方式： OAI收割
来源：武汉植物园
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