Investigation of benzylisoquinoline alkaloid biosynthetic pathway and its transcriptional regulation in lotus
文献类型：期刊论文
| 作者 | Ogutu, Collins1,2; Yang, Dong1; Liu, Yanling1; Vimolmangkang, Sornkanok4; Han, Yuepeng1,3; Deng, Xianbao1; Zhao, Li1,2; Fang, Ting1,2; Xiong, Yaqian1 |
| 刊名 | HORTICULTURE RESEARCH
 |
| 出版日期 | 2018-06-01 |
| 卷号 | 5页码:16 |
| ISSN号 | 2052-7276 |
| DOI | 10.1038/s41438-018-0035-0 |
| 英文摘要 | Lotus predominantly accumulates benzylisoquinoline alkaloids (BIAs), but their biosynthesis and regulation remain unclear. Here, we investigated structural and regulatory genes involved in BIA accumulation in lotus. Two clustered CYP80 genes were identified to be responsible for the biosynthesis of bis-BIAS and aporphine-type BIAs, respectively, and their tissue-specific expression causes divergence in alkaloid component between leaf and embryo. In contrast with the common (S)-reticuline precursor for most BIAs, aporphine alkaloids in lotus leaf may result from the (S)-Nmethylcoclaurine precursor. Structural diversity of BIA alkaloids in the leaf is attributed to enzymatic modifications, including intramolecular C-C phenol coupling on ring A and methylation and demethylation at certain positions. Additionally, most BIA biosynthetic pathway genes show higher levels of expression in the leaf of high-BIA cultivar compared with low-BIA cultivar, suggesting transcriptional regulation of BIA accumulation in lotus. Five transcription factors, including three MYBs, one ethylene-responsive factor, and one basic helix-loop-helix (bHLH), were identified to be candidate regulators of BIA biosynthesis in lotus. Our study reveals a BIA biosynthetic pathway and its transcriptional regulation in lotus, which will enable a deeper understanding of BIA biosynthesis in plants. |
| 资助项目 | National Natural Science Foundation of China[31450110420] ; National Natural Science Foundation of China[31272195] ; Overseas Construction Plan for Science and Education Base, China-Africa Center for Research and Education, Chinese Academy of Sciences[SAJC201327] |
| WOS研究方向 | Plant Sciences ; Agriculture |
| 语种 | 英语 |
| WOS记录号 | WOS:000433920100003 |
| 出版者 | NATURE PUBLISHING GROUP |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/5278]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Han, Yuepeng |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Plant Germplasrn Enhancement & Specialty, Wuhan Bot Garden, Wuhan 430074, Hubei, Peoples R China 2.Grad Univ Chinese Acad Sci, 19A Yuquanlu, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Sinoafrican Joint Res Ctr, Wuhan 430074, Hubei, Peoples R China 4.Chulalongkorn Univ, Fac Pharmaceut Sci, Dept Biochem & Microbiol, Bangkok 10330, Thailand |
| 推荐引用方式 GB/T 7714 | Ogutu, Collins,Yang, Dong,Liu, Yanling,et al. Investigation of benzylisoquinoline alkaloid biosynthetic pathway and its transcriptional regulation in lotus[J]. HORTICULTURE RESEARCH,2018,5:16. |
| APA | Ogutu, Collins.,Yang, Dong.,Liu, Yanling.,Vimolmangkang, Sornkanok.,Han, Yuepeng.,...&Xiong, Yaqian.(2018).Investigation of benzylisoquinoline alkaloid biosynthetic pathway and its transcriptional regulation in lotus.HORTICULTURE RESEARCH,5,16. |
| MLA | Ogutu, Collins,et al."Investigation of benzylisoquinoline alkaloid biosynthetic pathway and its transcriptional regulation in lotus".HORTICULTURE RESEARCH 5(2018):16. |
入库方式： OAI收割
来源：武汉植物园
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