An orthogonal system for heterologous expression of actinobacterial lasso peptides in Streptomyces hosts
文献类型：期刊论文
| 作者 | Zirah, Severine2; Afonso, Carlos4; Zotchev, Sergey B.1; Rebuffat, Sylvie2; Li, Yanyan2; Mevaere, Jimmy2; Goulard, Christophe2; Schneider, Olha3; Sekurova, Olga N.1; Ma, Haiyan2,5 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2018-05-29 |
| 卷号 | 8页码:11 |
| ISSN号 | 2045-2322 |
| DOI | 10.1038/s41598-018-26620-0 |
| 英文摘要 | Lasso peptides are ribosomally synthesized and post-translationally modified peptides produced by bacteria. They are characterized by an unusual lariat-knot structure. Targeted genome scanning revealed a wide diversity of lasso peptides encoded in actinobacterial genomes, but cloning and heterologous expression of these clusters turned out to be problematic. To circumvent this, we developed an orthogonal expression system for heterologous production of actinobacterial lasso peptides in Streptomyces hosts based on a newly-identified regulatory circuit from Actinoalloteichus fjordicus. Six lasso peptide gene clusters, mainly originating from marine Actinobacteria, were chosen for proof-of-concept studies. By varying the Streptomyces expression hosts and a small set of culture conditions, three new lasso peptides were successfully produced and characterized by tandem MS. The newly developed expression system thus sets the stage to uncover and bioengineer the chemodiversity of actinobacterial lasso peptides. Moreover, our data provide some considerations for future bioprospecting efforts for such peptides. |
| 资助项目 | European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH[613877] ; University of Vienna ; NTNU ; City of Paris |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000433290000011 |
| 出版者 | NATURE PUBLISHING GROUP |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/5318]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Zotchev, Sergey B.; Li, Yanyan |
| 作者单位 | 1.Univ Vienna, Dept Pharmacognosy, Althanstr 14, A-1090 Vienna, Austria 2.Sorbonne Univ, CNRS, Lab Mol Commun & Adaptat Microorganisms, MCAM,UMR 7245,MNHN, CP 54,57 Rue Cuvier, F-75005 Paris, France 3.Norwegian Univ Sci & Technol NTNU, Dept Biotechnol, N-7491 Trondheim, Norway 4.Normandie Univ, CNRS, UNIROUEN, INSA Rouen,COBRA, Rouen, France 5.Chinese Acad Sci, Inst Hydrobiol, Ctr Microalgal Biotechnol & Biofuels, Wuhan, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zirah, Severine,Afonso, Carlos,Zotchev, Sergey B.,et al. An orthogonal system for heterologous expression of actinobacterial lasso peptides in Streptomyces hosts[J]. SCIENTIFIC REPORTS,2018,8:11. |
| APA | Zirah, Severine.,Afonso, Carlos.,Zotchev, Sergey B..,Rebuffat, Sylvie.,Li, Yanyan.,...&Ma, Haiyan.(2018).An orthogonal system for heterologous expression of actinobacterial lasso peptides in Streptomyces hosts.SCIENTIFIC REPORTS,8,11. |
| MLA | Zirah, Severine,et al."An orthogonal system for heterologous expression of actinobacterial lasso peptides in Streptomyces hosts".SCIENTIFIC REPORTS 8(2018):11. |
入库方式： OAI收割
来源：武汉植物园
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