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RBM24 stabilizes hepatitis B virus pregenomic RNA but inhibits core protein translation by targeting the terminal redundancy sequence

文献类型:期刊论文

AuthorWang, Yun2; Zhao, Kaitao2,4; Yuan, Yifei2,4; Chen, Yingshan2,4; Zhang, Zhenhua1,3; Pei, Rongjuan2; Chen, Jizheng2; Hu, Xue2; Zhou, Yuan2; Lu, Mengji5
SourceEMERGING MICROBES & INFECTIONS
Issued Date2018-05-14
Volume7Pages:14
ISSN2222-1751
DOI10.1038/s41426-018-0091-4
English AbstractThe terminal redundancy (TR) sequence of the 3.5-kb hepatitis B virus (HBV) RNA contains sites that govern many crucial functions in the viral life cycle, including polyadenylation, translation, RNA packaging, and DNA synthesis. In the present study, RNA-binding motif protein 24 (RBM24) is shown to be involved in the modulation of HBV replication by targeting the TR of HBV RNA. In HBV-transfected hepatoma cell lines, both knockdown and overexpression of RBM24 led to decreased HBV replication and transcription. Ectopic expression of RBM24 inhibited HBV replication, which was partly restored by knockdown of RBM24, indicating that a proper level of RBM24 was required for HBV replication. The regulation of RBM24 of HBV replication and translation was achieved by the interaction between the RNA-binding domains of RBM24 and both the 5' and 3' TR of 3.5-kb RNA. RBM24 interacted with the 5' TR of HBV pregenomic RNA (pgRNA) to block 80S ribosome assembly on HBV pgRNA and thus inhibited core protein translation, whereas the interaction between RBM24 and the 3' TR enhanced the stability of HBV RNA. Finally, the regulatory function of RBM24 on HBV replication was further confirmed in a HBV infection model. In conclusion, the present study demonstrates the dual functions of RBM24 by interacting with different TRs of viral RNA and reveals that RBM24 is an important host gene for HBV replication.
Funding ProjectNational Nature Science Foundation of China[31770180] ; National Nature Science Foundation of China[31621061] ; Youth Innovation Promotion Association CAS[201603]
WOS Research AreaImmunology ; Microbiology
Language英语
PublisherNATURE PUBLISHING GROUP
WOS IDWOS:000432753300001
源URL[http://202.127.146.157/handle/2RYDP1HH/5359]  
Collection中国科学院武汉植物园
Corresponding AuthorWu, Chunchen; Chen, Xinwen
Affiliation1.Anhui Med Univ, Sch Pharm, Hefei 230022, Anhui, Peoples R China
2.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan, Hubei, Peoples R China
3.Anhui Med Univ, Affiliated Hosp 1, Dept Infect Dis, Hefei 230022, Anhui, Peoples R China
4.Univ Chinese Acad Sci, Beijing, Peoples R China
5.Univ Hosp Essen, Inst Virol, Essen, Germany
Recommended Citation
GB/T 7714
Wang, Yun,Zhao, Kaitao,Yuan, Yifei,et al. RBM24 stabilizes hepatitis B virus pregenomic RNA but inhibits core protein translation by targeting the terminal redundancy sequence[J]. EMERGING MICROBES & INFECTIONS,2018,7:14.
APA Wang, Yun.,Zhao, Kaitao.,Yuan, Yifei.,Chen, Yingshan.,Zhang, Zhenhua.,...&Cao, Huang.(2018).RBM24 stabilizes hepatitis B virus pregenomic RNA but inhibits core protein translation by targeting the terminal redundancy sequence.EMERGING MICROBES & INFECTIONS,7,14.
MLA Wang, Yun,et al."RBM24 stabilizes hepatitis B virus pregenomic RNA but inhibits core protein translation by targeting the terminal redundancy sequence".EMERGING MICROBES & INFECTIONS 7(2018):14.

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来源:武汉植物园

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