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RBM24 stabilizes hepatitis B virus pregenomic RNA but inhibits core protein translation by targeting the terminal redundancy sequence

文献类型：期刊论文

作者Wang, Yun2; Zhao, Kaitao2,4; Yuan, Yifei2,4; Chen, Yingshan2,4; Zhang, Zhenhua1,3; Pei, Rongjuan2; Chen, Jizheng2; Hu, Xue2; Zhou, Yuan2; Lu, Mengji5
刊名EMERGING MICROBES & INFECTIONS
出版日期2018-05-14
卷号7页码:14
ISSN号2222-1751
DOI10.1038/s41426-018-0091-4
英文摘要The terminal redundancy (TR) sequence of the 3.5-kb hepatitis B virus (HBV) RNA contains sites that govern many crucial functions in the viral life cycle, including polyadenylation, translation, RNA packaging, and DNA synthesis. In the present study, RNA-binding motif protein 24 (RBM24) is shown to be involved in the modulation of HBV replication by targeting the TR of HBV RNA. In HBV-transfected hepatoma cell lines, both knockdown and overexpression of RBM24 led to decreased HBV replication and transcription. Ectopic expression of RBM24 inhibited HBV replication, which was partly restored by knockdown of RBM24, indicating that a proper level of RBM24 was required for HBV replication. The regulation of RBM24 of HBV replication and translation was achieved by the interaction between the RNA-binding domains of RBM24 and both the 5' and 3' TR of 3.5-kb RNA. RBM24 interacted with the 5' TR of HBV pregenomic RNA (pgRNA) to block 80S ribosome assembly on HBV pgRNA and thus inhibited core protein translation, whereas the interaction between RBM24 and the 3' TR enhanced the stability of HBV RNA. Finally, the regulatory function of RBM24 on HBV replication was further confirmed in a HBV infection model. In conclusion, the present study demonstrates the dual functions of RBM24 by interacting with different TRs of viral RNA and reveals that RBM24 is an important host gene for HBV replication.
资助项目National Nature Science Foundation of China[31770180] ; National Nature Science Foundation of China[31621061] ; Youth Innovation Promotion Association CAS[201603]
WOS研究方向Immunology ; Microbiology
语种英语
出版者NATURE PUBLISHING GROUP
WOS记录号WOS:000432753300001
源URL[http://202.127.146.157/handle/2RYDP1HH/5359]  
专题中国科学院武汉植物园
通讯作者Wu, Chunchen; Chen, Xinwen
作者单位1.Anhui Med Univ, Sch Pharm, Hefei 230022, Anhui, Peoples R China
2.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan, Hubei, Peoples R China
3.Anhui Med Univ, Affiliated Hosp 1, Dept Infect Dis, Hefei 230022, Anhui, Peoples R China
4.Univ Chinese Acad Sci, Beijing, Peoples R China
5.Univ Hosp Essen, Inst Virol, Essen, Germany
推荐引用方式
GB/T 7714		 Wang, Yun,Zhao, Kaitao,Yuan, Yifei,et al. RBM24 stabilizes hepatitis B virus pregenomic RNA but inhibits core protein translation by targeting the terminal redundancy sequence[J]. EMERGING MICROBES & INFECTIONS,2018,7:14.
APA Wang, Yun.,Zhao, Kaitao.,Yuan, Yifei.,Chen, Yingshan.,Zhang, Zhenhua.,...&Cao, Huang.(2018).RBM24 stabilizes hepatitis B virus pregenomic RNA but inhibits core protein translation by targeting the terminal redundancy sequence.EMERGING MICROBES & INFECTIONS,7,14.
MLA Wang, Yun,et al."RBM24 stabilizes hepatitis B virus pregenomic RNA but inhibits core protein translation by targeting the terminal redundancy sequence".EMERGING MICROBES & INFECTIONS 7(2018):14.

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来源：武汉植物园

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