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Proteomic Analysis of Zika Virus Infected Primary Human Fetal Neura Progenitors Suggests a Role for Doublecortin in the Pathological Consequences of Infection in the Cortex

文献类型：期刊论文

作者Tang, Ya-Ping1; Yuan, Hui9; Gao, George F.4,6,7,8; Luo, Min-Hua1,2,3,7; Jiang, Xuan1,2; Dong, Xiao3; Li, Shi-Hua4; Zhou, Yue-Peng3; Rayner, Simon5; Xia, Hui-Min1
刊名FRONTIERS IN MICROBIOLOGY
出版日期2018-06-05
卷号9页码:15
关键词Zika virus human fetal neural progenitor cells (NPCs) doublecortin (DCX) cortex structure proteomic analysis
ISSN号1664-302X
DOI10.3389/fmicb.2018.01067
英文摘要Zika virus (ZIKV) infection is associated with severe neurological defects in fetuses and newborns, such as microcephaly. However, the underlying mechanisms remain to be elucidated. In this study, proteomic analysis on ZIKV-infected primary human fetal neural progenitor cells (NPCs) revealed that virus infection altered levels of cellular proteins involved in NPC proliferation, differentiation and migration. The transcriptional levels of some of the altered targets were also confirmed by qRT-PCR. Among the altered proteins, doublecortin (DCX) plays an important role in NPC differentiation and migration. Results showed that ZIKV infection downregulated DCX, at both mRNA and protein levels, as early as 1 day post infection (1 dpi), and lasted throughout the virus replication cycle (4 days). The downregulation of DCX was also observed in a ZIKV-infected fetal mouse brain model, which displayed decreased body weight, brain size and weight, as well as defective cortex structure. By screening the ten viral proteins of ZIKV, we found that both the expression of NS4A and NS5 were correlated with the downregulation of both mRNA and protein levels of DCX in NPCs. These data suggest that DCX is modulated following infection of the brain by ZIKV. How these observed changes of DCX expression translate in the pathological consequences of ZIKV infection and if other cellular proteins are equally involved remains to be investigated.
资助项目National Key Research and Development Program of China[2016YFC1200400] ; Major Program of Guangzhou healthcare collaborative innovation foundation[201704020229] ; Sino-Africa joint research center, Chinese Academy of Sciences[SAJC201605] ; National Nature Science Foundation of China[81571355] ; National Nature Science Foundation of China[81620108021]
WOS研究方向Microbiology
语种英语
WOS记录号WOS:000434136800001
出版者FRONTIERS MEDIA SA
源URL[http://202.127.146.157/handle/2RYDP1HH/5386]  
专题中国科学院武汉植物园
通讯作者Tang, Ya-Ping; Yuan, Hui; Luo, Min-Hua
作者单位1.Guangzhou Women & Children Med Ctr, Guangzhou Inst Pediat, Joint Ctr Translat Precis Med, Guangzhou, Guangdong, Peoples R China
2.Chinese Acad Sci, Wuhan Inst Virol, Joint Ctr Translat Precis Med, Wuhan, Hubei, Peoples R China
3.Chinese Acad Sci, Wuhan Inst Virol, CAS Ctr Excellence Brain Sci & Intelligence Techn, State Key Lab Virol, Wuhan, Hubei, Peoples R China
4.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Pathogen Microbiol & Immunol, Beijing, Peoples R China
5.Univ Oslo, Oslo Univ Hosp, Dept Med Genet, Oslo, Norway
6.Natl Inst Viral Dis Control & Prevent, Chinese Ctr Dis Control & Prevent, Beijing, Peoples R China
7.Univ Chinese Acad Sci, Savaid Med Sch, Beijing, Peoples R China
8.Chinese Acad Sci, Beijing Inst Life Sci, Res Network Immun & Hlth, Beijing, Peoples R China
9.Jianghan Univ, Med Coll, Dept Med, Wuhan, Hubei, Peoples R China
推荐引用方式
GB/T 7714		 Tang, Ya-Ping,Yuan, Hui,Gao, George F.,et al. Proteomic Analysis of Zika Virus Infected Primary Human Fetal Neura Progenitors Suggests a Role for Doublecortin in the Pathological Consequences of Infection in the Cortex[J]. FRONTIERS IN MICROBIOLOGY,2018,9:15.
APA Tang, Ya-Ping.,Yuan, Hui.,Gao, George F..,Luo, Min-Hua.,Jiang, Xuan.,...&Xia, Hui-Min.(2018).Proteomic Analysis of Zika Virus Infected Primary Human Fetal Neura Progenitors Suggests a Role for Doublecortin in the Pathological Consequences of Infection in the Cortex.FRONTIERS IN MICROBIOLOGY,9,15.
MLA Tang, Ya-Ping,et al."Proteomic Analysis of Zika Virus Infected Primary Human Fetal Neura Progenitors Suggests a Role for Doublecortin in the Pathological Consequences of Infection in the Cortex".FRONTIERS IN MICROBIOLOGY 9(2018):15.

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