中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Discovery of Novel Bat Coronaviruses in South China That Use the Same Receptor as Middle East Respiratory Syndrome Coronavirus

文献类型:期刊论文

作者Wang, Ning2,3; Yang, Xing-Lou2; Liu, Hai-Zhou2; Zhang, Wei2; Li, Bei2; Hu, Ben2; Peng, Cheng2; Zhu, Guang-Jian4; Geng, Qi-Bin1; Li, Fang1
刊名JOURNAL OF VIROLOGY
出版日期2018-07-01
卷号92期号:13页码:15
ISSN号0022-538X
关键词MERS-related coronavirus bat dipeptidyl peptidase 4 virus discovery
DOI10.1128/JVI.00116-18
英文摘要Middle East respiratory syndrome coronavirus (MERS-CoV) has represented a human health threat since 2012. Although several MERS-related CoVs that belong to the same species as MERS-CoV have been identified from bats, they do not use the MERS-CoV receptor, dipeptidyl peptidase 4 (DPP4). Here, we screened 1,059 bat samples from at least 30 bat species collected in different regions in south China and identified 89 strains of lineage C betacoronaviruses, including Tylonycteris pachypus coronavirus HKU4, Pipistrellus pipistrellus coronavirus HKU5, and MERSrelated CoVs. We sequenced the full-length genomes of two positive samples collected from the great evening bat, Ia io, from Guangdong Province. The two genomes were highly similar and exhibited genomic structures identical to those of other lineage C betacoronaviruses. While they exhibited genome-wide nucleotide identities of only 75.3 to 81.2% with other MERS-related CoVs, their gene-coding regions were highly similar to their counterparts, except in the case of the spike proteins. Further protein-protein interaction assays demonstrated that the spike proteins of these MERS-related CoVs bind to the receptor DPP4. Recombination analysis suggested that the newly discovered MERS-related CoVs have acquired their spike genes from a DPP4-recognizing bat coronavirus HKU4. Our study provides further evidence that bats represent the evolutionary origins of MERS-CoV. IMPORTANCE Previous studies suggested that MERS-CoV originated in bats. However, its evolutionary path from bats to humans remains unclear. In this study, we discovered 89 novel lineage C betacoronaviruses in eight bat species. We provide evidence of a MERS-related CoV derived from the great evening bat that uses the same host receptor as human MERS-CoV. This virus also provides evidence for a natural recombination event between the bat MERS-related CoV and another bat coronavirus, HKU4. Our study expands the host ranges of MERS-related CoV and represents an important step toward establishing bats as the natural reservoir of MERS-CoV. These findings may lead to improved epidemiological surveillance of MERS-CoV and the prevention and control of the spread of MERS-CoV to humans.
资助项目Scientific and Technological Basis Special Project from the Ministry of Science and Technology of the People's Republic of China[2013FY113500] ; USAID Emerging Pandemic Threats PREDICT project grant[AID-OAA-A-14-00102] ; NIH[R01AI110964] ; NIH[R01AI089728] ; NIH[R01AI110700] ; National Natural Science Foundation of China[31727901]
WOS研究方向Virology
语种英语
出版者AMER SOC MICROBIOLOGY
WOS记录号WOS:000435100400005
源URL[http://202.127.146.157/handle/2RYDP1HH/5407]  
专题中国科学院武汉植物园
通讯作者Li, Fang; Shi, Zheng-Li
作者单位1.Univ Minnesota, Dept Vet & Biomed Sci, St Paul, MN 55108 USA
2.Chinese Acad Sci, Wuhan Inst Virol, CAS Key Lab Special Pathogens & Biosafety, Wuhan, Hubei, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
4.EcoHlth Alliance, New York, NY USA
推荐引用方式
GB/T 7714
Wang, Ning,Yang, Xing-Lou,Liu, Hai-Zhou,et al. Discovery of Novel Bat Coronaviruses in South China That Use the Same Receptor as Middle East Respiratory Syndrome Coronavirus[J]. JOURNAL OF VIROLOGY,2018,92(13):15.
APA Wang, Ning.,Yang, Xing-Lou.,Liu, Hai-Zhou.,Zhang, Wei.,Li, Bei.,...&Luo, Chu-Ming.(2018).Discovery of Novel Bat Coronaviruses in South China That Use the Same Receptor as Middle East Respiratory Syndrome Coronavirus.JOURNAL OF VIROLOGY,92(13),15.
MLA Wang, Ning,et al."Discovery of Novel Bat Coronaviruses in South China That Use the Same Receptor as Middle East Respiratory Syndrome Coronavirus".JOURNAL OF VIROLOGY 92.13(2018):15.

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来源:武汉植物园

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