Three amino acid substitutions in the NS1 protein change the virus replication of H5N1 influenza virus in human cells
文献类型：期刊论文
| 作者 | Chen, Quanjiao5; Li, Jing1,2; Zhang, Kun6; Zhang, Xiaoshuang5; Sun, Yeping2; Bi, Yuhai2; Zhang, Shuang2; Gu, Jinyan1; Li, Jiarong1; Liu, Di2,5 |
| 刊名 | VIROLOGY
 |
| 出版日期 | 2018-06-01 |
| 卷号 | 519页码:64-73 |
| 关键词 | H5N1 influenza virus NS1 protein IFN response Virus replication |
| ISSN号 | 0042-6822 |
| DOI | 10.1016/j.virol.2018.04.004 |
| 英文摘要 | Influenza A viruses have sophisticated strategies to promote their own replication. Here, we found that three H5N1 influenza viruses display different replication patterns in human A549 and macrophage cells. The HN01 virus displayed poor replication compared to HN021 and JS01. In addition, the HN01 virus was unable to counteract the interferon response and block general gene expression. This capability was restored by three amino acid substitutions on the NS1 protein: K55E, K66E, and C133F, resulting in recovered binding to CPSF30 and decreased interferon response activity. Furthermore, a recombinant HN01 virus expressing either NS1-C133F or the triple mutation replicate with higher titers in human A549 cells and macrophages compared to the parent virus. These three amino acid mutations reveal a new pathway to alter H5N1 virus replication. |
| 资助项目 | National Key Research Program[2016YFD0500206] ; National Key Research Program[2016YFC1200803] ; National Key Research Program[2017YFD051105] ; National Key Research Program[2016YFC1201303] ; National Natural Science Foundation of China (NSFC)[31630079] ; National Natural Science Foundation of China (NSFC)[31572526] ; Southeast Asia Biodiversity Research Institute, Chinese Academy of Science[Y4ZK111B01] ; Strategic Priority Research Program of Chinese Academy of Sciences[XDPB03] ; Innovative Research Group of National Natural Science Foundation of China[81621091] |
| WOS研究方向 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000435228000008 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/5485]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Liu, Wenjun; Zhou, Jiyong |
| 作者单位 | 1.Nanjing Agr Univ, MOE Joint Int Res Lab Anim Immunol, Nanjing, Jiangsu, Peoples R China 2.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Pathogen Microbiol & Immunol, Beijing, Peoples R China 3.Zhejiang Univ, Key Lab Anim Virol, Minist Agr, Hangzhou, Zhejiang, Peoples R China 4.Zhejiang Univ, Affiliated Hosp 1, Collaborat Innovat Ctr Diag & Treatment Infect Di, Hangzhou, Zhejiang, Peoples R China 5.Chinese Acad Sci, Wuhan Inst Virol, CAS Key Lab Special Pathogens & Biosafety, Wuhan, Hubei, Peoples R China 6.Virginia Commonwealth Univ, Sch Dent, Philips Inst Oral Hlth Res, Richmond, VA USA |
| 推荐引用方式 GB/T 7714 | Chen, Quanjiao,Li, Jing,Zhang, Kun,et al. Three amino acid substitutions in the NS1 protein change the virus replication of H5N1 influenza virus in human cells[J]. VIROLOGY,2018,519:64-73. |
| APA | Chen, Quanjiao.,Li, Jing.,Zhang, Kun.,Zhang, Xiaoshuang.,Sun, Yeping.,...&Zhou, Jiyong.(2018).Three amino acid substitutions in the NS1 protein change the virus replication of H5N1 influenza virus in human cells.VIROLOGY,519,64-73. |
| MLA | Chen, Quanjiao,et al."Three amino acid substitutions in the NS1 protein change the virus replication of H5N1 influenza virus in human cells".VIROLOGY 519(2018):64-73. |
入库方式： OAI收割
来源：武汉植物园
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