A Microfluidic Fluorescent Flow Cytometry Capable of Quantifying Cell Sizes and Numbers of Specific Cytosolic Proteins
文献类型：期刊论文
| 作者 | Chen, Jian2,3; Wang, Junbo2,3; Men, Dong1; Cao, Shanshan1; Chen, Deyong2,3; Liu, Lixing2,3; Fan, Beiyuan2,3; Li, Xiufeng2,3 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2018-09-21 |
| 卷号 | 8页码:9 |
| ISSN号 | 2045-2322 |
| DOI | 10.1038/s41598-018-32333-1 |
| 英文摘要 | This study presents a microfluidics based cytometry capable of characterizing cell sizes and counting numbers of specific cytosolic proteins where cells were first bound by antibodies labelled with fluorescence and then aspirated into a constriction microchannel in which fluorescent levels were measured. These raw fluorescent pulses were further divided into a rising domain, a stable domain and a declining domain. In addition, antibody solutions with labelled fluorescence were aspirated through the constriction microchannel, yielding curves to translate raw fluorescent levels to protein concentrations. By using key parameters of three domains as well as the calibration curves, cell diameters and the absolute number of beta-actins at the single-cell level were quantified as 14.2 +/- 1.7 mu m and 9.62 +/- 4.29 X 10(5) (A549, n(cell) = 14 242), 13.0 +/- 2.0 mu m and 6.46 +/- 3.34 X 10(5) (Hep G2, n(cell) = 35 932), 13.8 +/- 1.9 mu m and 1.58 +/- 0.90 X 10(6) (MCF 10A, n(cell) = 16 650), and 12.7 +/- 1.5 mu m and 1.09 +/- 0.49 X 10(6) (HeLa, n(cell) = 26 246). This platform could be further adopted to measure numbers of various cytosolic proteins, providing key insights in proteomics at the single-cell level. |
| 资助项目 | 973 Program[2014CB744600] ; NSFC[61431019] ; NSFC[61671430] ; Key Project of CAS[QYZDB-SSW-JSC011] ; Instrument Program of CAS ; Youth Innovation Promotion Association of CAS ; CAS Interdisciplinary Innovation Team |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000445276900003 |
| 出版者 | NATURE PUBLISHING GROUP |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/5715]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Chen, Jian; Wang, Junbo; Men, Dong |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Virol, Wuhan Inst Virol, Wuhan, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Acad Sci, State Key Lab Transducer Technol, Inst Elect, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Jian,Wang, Junbo,Men, Dong,et al. A Microfluidic Fluorescent Flow Cytometry Capable of Quantifying Cell Sizes and Numbers of Specific Cytosolic Proteins[J]. SCIENTIFIC REPORTS,2018,8:9. |
| APA | Chen, Jian.,Wang, Junbo.,Men, Dong.,Cao, Shanshan.,Chen, Deyong.,...&Li, Xiufeng.(2018).A Microfluidic Fluorescent Flow Cytometry Capable of Quantifying Cell Sizes and Numbers of Specific Cytosolic Proteins.SCIENTIFIC REPORTS,8,9. |
| MLA | Chen, Jian,et al."A Microfluidic Fluorescent Flow Cytometry Capable of Quantifying Cell Sizes and Numbers of Specific Cytosolic Proteins".SCIENTIFIC REPORTS 8(2018):9. |
入库方式： OAI收割
来源：武汉植物园
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