Two amino acid changes in the R3 repeat cause functional divergence of two clustered MYB10 genes in peach
文献类型：期刊论文
| 作者 | Han, Yuepeng1,5; Jiang, Quan3; Wang, Lu5; Ogutu, Collins4,5; Zhao, Jianbo3; Ren, Fei3; Xu, Shengli4,5; Liao, Liao5; Zhou, Hui2,5 |
| 刊名 | PLANT MOLECULAR BIOLOGY
 |
| 出版日期 | 2018-09-01 |
| 卷号 | 98期号:1-2页码:169-183 |
| 关键词 | Prunus Anthocyanin coloration R2R3-MYB TF bHLH gene DNA-binding affinity Site-directed mutation |
| ISSN号 | 0167-4412 |
| DOI | 10.1007/s11103-018-0773-2 |
| 英文摘要 | R2R3-MYB genes play a pivotal role in regulating anthocyanin accumulation. Here, we report two tandemly duplicated R2R3-MYB genes in peach, PpMYB10.1 and PpMYB10.2, with the latter showing lower ability to induce anthocyanin accumulation than the former. Site-directed mutation assay revealed two amino acid changes in the R3 repeat, Arg/Lys(66) and Gly/Arg(93), responsible for functional divergence between these two PpMYB10 genes. Anthocyanin-promoting activity of PpMYB10.2 was significantly increased by a single amino acid replacement of Arg(93) with Gly(93). However, either the Gly(93) Arg(93) or Arg(66) Lys(66) substitutions alone showed little impact on anthocyanin-promoting activity of PpMYB10.1, but simultaneous substitutions caused a significant decrease. Reciprocal substitution of Arg/Gly(93) could significantly alter binding affinity to PpbHLH3, while the Arg(66) Lys(66) substitution is predicted to affect the folding of the MYB DNA-binding domain, instead of PpbHLH3-binding affinity. Overall, the change of anthocyanin-promoting activity was accompanied with that of bHLH-binding affinity, suggesting that DNA-binding affinity of R2R3-MYBs depends on their bHLH partners. Our study is helpful for understanding of functional evolution of R2R3-MYBs and their interaction with DNA targets. |
| 资助项目 | National Natural Science Foundation of China[31672134] ; China Agriculture Research System[CARS-30] ; Overseas Construction Plan for Science and Education Base, China-Africa Center for Research and Education, Chinese Academy of Sciences[SAJC201327] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000445210900011 |
| 出版者 | SPRINGER |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/5723]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Han, Yuepeng |
| 作者单位 | 1.Chinese Acad Sci, Sinoafrican Joint Res Ctr, Wuhan 430074, Hubei, Peoples R China 2.Anhui Acad Agr Sci, Hort Inst, Key Lab Genet Improvement & Ecophysiol Hort Crops, Hefei 230031, Anhui, Peoples R China 3.Beijing Acad Agr & Forestry Sci, Inst Forestry & Pomol, Beijing 100097, Peoples R China 4.Univ Chinese Acad Sci, 19A Yuquanlu, Beijing 100049, Peoples R China 5.Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan Bot Garden, Wuhan 430074, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Han, Yuepeng,Jiang, Quan,Wang, Lu,et al. Two amino acid changes in the R3 repeat cause functional divergence of two clustered MYB10 genes in peach[J]. PLANT MOLECULAR BIOLOGY,2018,98(1-2):169-183. |
| APA | Han, Yuepeng.,Jiang, Quan.,Wang, Lu.,Ogutu, Collins.,Zhao, Jianbo.,...&Zhou, Hui.(2018).Two amino acid changes in the R3 repeat cause functional divergence of two clustered MYB10 genes in peach.PLANT MOLECULAR BIOLOGY,98(1-2),169-183. |
| MLA | Han, Yuepeng,et al."Two amino acid changes in the R3 repeat cause functional divergence of two clustered MYB10 genes in peach".PLANT MOLECULAR BIOLOGY 98.1-2(2018):169-183. |
入库方式： OAI收割
来源：武汉植物园
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