Transcript-associated microsatellites from gibel carpand their applicability of genetic analyses in Carassius auratus populations
文献类型：期刊论文
| 作者 | Tong, Jingou2; Yin, Zhan2; Gui, Jianfang2; Wang, Zhongwei2; Zhou, Li2; Yu, Xiaomu2; Gan, Baojiang2; Wang, Xinhua1,2 |
| 刊名 | JOURNAL OF APPLIED ICHTHYOLOGY
 |
| 出版日期 | 2018-10-01 |
| 卷号 | 34期号:5页码:1108-1116 |
| 关键词 | cross-strain amplification Gibel carp microsatellites polymorphism population genetic analyses |
| ISSN号 | 0175-8659 |
| DOI | 10.1111/jai.13762 |
| 英文摘要 | Microsatellites are popular co-dominant markers for different genetic analyses, and transcriptome sequencing provides a new approach for rapid development of microsatellite markers. Gibel carp is a triploid gynogenetic fish with great growth potentials, but no transcript-associated microsatellite markers are available. Here we developed and characterized a set of EST-SSRs from the transcriptome data of gibel carp. 126 out of the 150 EST-SSRs were successfully developed and 110 showed polymorphisms in gibel carp. Twenty-four randomly selected polymorphic EST-SSRs were characterized in a cultured population of gibel carp, and the number of alleles per locus ranged from 3 to 9 (mean 6.5), with an average proportion of heterozygotes of 84.2%. All 24 EST-SSRs were successfully amplified in a goldfish family, and genotypes of all loci were in the Mendelian expectations after Bonferroni corrections. In addition, seven polymorphic EST-SSRs were selected for genetic diversity analysis in seven wild crucian carp populations of China, and the results showed that these loci have abundant polymorphisms with allele numbers of 13-37. Taken together with the genetic variations of crucian carp populations, the average number of alleles (N-a) was 20.9 per locus, and the mean H-o, H-e and PIC were 0.752, 0.878 and 0.865, respectively. Structure analysis and clustering results consistently supported that seven crucian carp populations could be divided into two major branches, and the genetic distances are basically positively correlated with geographical distances. These transcript-derived microsatellites are valuable resources and powerful tools for population genetics and many other genetic analyses in gibel carp and its close relatives. |
| 资助项目 | Special Fund for Strategic Pilot Technology of CAS[XDA08010405] ; State Key Laboratory of Freshwater Ecology and Biotechnology of China[2016FBZ05] |
| WOS研究方向 | Fisheries ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000444542800002 |
| 出版者 | WILEY |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/5754]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Tong, Jingou |
| 作者单位 | 1.Henan Univ Anim Husb & Econ, Coll Anim Sci & Technol, Zhengzhou, Henan, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tong, Jingou,Yin, Zhan,Gui, Jianfang,et al. Transcript-associated microsatellites from gibel carpand their applicability of genetic analyses in Carassius auratus populations[J]. JOURNAL OF APPLIED ICHTHYOLOGY,2018,34(5):1108-1116. |
| APA | Tong, Jingou.,Yin, Zhan.,Gui, Jianfang.,Wang, Zhongwei.,Zhou, Li.,...&Wang, Xinhua.(2018).Transcript-associated microsatellites from gibel carpand their applicability of genetic analyses in Carassius auratus populations.JOURNAL OF APPLIED ICHTHYOLOGY,34(5),1108-1116. |
| MLA | Tong, Jingou,et al."Transcript-associated microsatellites from gibel carpand their applicability of genetic analyses in Carassius auratus populations".JOURNAL OF APPLIED ICHTHYOLOGY 34.5(2018):1108-1116. |
入库方式： OAI收割
来源：武汉植物园
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