Molecular characterization of the giant freshwater prawn (Macrobrachium rosenbergii) beta-actin gene promoter
文献类型：期刊论文
| 作者 | Long, Yong4; Yang, Guoliang1; Cui, Zongbin4; Gao, Qiang2; Yan, Junjun3,4 |
| 刊名 | PEERJ
 |
| 出版日期 | 2018-10-23 |
| 卷号 | 6页码:18 |
| 关键词 | Giant freshwater prawn Beta-actin gene Promoter Transcriptional activity |
| ISSN号 | 2167-8359 |
| DOI | 10.7717/peerj.5701 |
| 英文摘要 | Constitutive promoters are important tools for gene function studies and transgenesis. The Beta-actin (actb1) gene promoter has been isolated from many species but remains to be cloned from the giant freshwater prawn (Macrobrachium rosenbergii). In this study, we cloned and characterized the Mractb1 gene promoter. Two alternative promoters were identified for the Mractb1 gene, which direct the generation of two transcripts with different 5' untranslated regions. Three CpG islands were predicted in the upstream sequence, which are intimately related to transcription initiation and promoter activity. In addition to the CCAAT-box and the CArG-box, molecular dissection of the flanking sequence revealed the existence of one negative and two positive elements in the upstream region and the first intron. Finally, the Mractb1 promoter demonstrated comparative activity to the carp (Cyprinus carpio) actb1 promoter. Our investigations provide a valuable genetic tool for gene function studies and shed light on the regulation of the Mractb1 gene. |
| 资助项目 | Youth Innovation Promotion Association of Chinese Academy of Sciences[2016305] ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province[2012C12907-2] |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000448082800004 |
| 出版者 | PEERJ INC |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/5852]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Long, Yong; Yang, Guoliang |
| 作者单位 | 1.Huzhou Univ, Huzhou, Peoples R China 2.Zhejiang Inst Freshwater Fisheries, Huzhou, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Long, Yong,Yang, Guoliang,Cui, Zongbin,et al. Molecular characterization of the giant freshwater prawn (Macrobrachium rosenbergii) beta-actin gene promoter[J]. PEERJ,2018,6:18. |
| APA | Long, Yong,Yang, Guoliang,Cui, Zongbin,Gao, Qiang,&Yan, Junjun.(2018).Molecular characterization of the giant freshwater prawn (Macrobrachium rosenbergii) beta-actin gene promoter.PEERJ,6,18. |
| MLA | Long, Yong,et al."Molecular characterization of the giant freshwater prawn (Macrobrachium rosenbergii) beta-actin gene promoter".PEERJ 6(2018):18. |
入库方式： OAI收割
来源：武汉植物园
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