Positive and Negative Regulation of Glycerol Utilization by the c-di-GMP Binding Protein PIzA in Borrelia burgdorferi
文献类型:期刊论文
作者 | Yang, X. Frank6; Gomelsky, Mark3; Carrasco, Sebastian E.1,6; He, Ming6; Troxell, Bryan2,6; Li, Hongxia6; Du, Jimei4,6; Yang, Youyun6; Chen, Tong4,6; Zhang, Jun-Jie5,6 |
刊名 | JOURNAL OF BACTERIOLOGY |
出版日期 | 2018-11-01 |
卷号 | 200期号:22页码:11 |
ISSN号 | 0021-9193 |
关键词 | Borrelia burgdorferi Lyme disease PIzA tick-borne pathogens |
DOI | 10.1128/JB.00243-18 |
英文摘要 | Borrelia burgdorferi, the causative agent of Lyme disease, encounters two disparate host environments during its enzootic life cycle, &odes ticks and mammalian hosts. B. burgdorferi has a small genome that encodes a streamlined cyclic dimeric GMP (c-di-GMP) signaling system comprising a single diguanylate cyclase, Rrpl, and two phosphodiesterases. This system is essential for spirochete survival in ticks, in part because it controls the expression of the glp operon involved in glycerol utilization. In this study, we showed that a B. burgdorferi c-di-GMP receptor, PIzA, functions as both a positive and a negative regulator for glp expression. Deletion of pizA or mutation in pizA that impaired c-di-GMP binding abolished glp expression. On the other hand, overexpression of pizA resulted in gip repression, which could be rescued by simultaneous overexpression of rip!. pizA overexpression in the rrpl mutant, which is devoid of c-di-GMP, or overexpression of a pizA mutant incapable of c-di-GMP binding further enhanced gip repression. Combined results suggest that c-di-GMP-bound PIzA functions as a positive regulator, whereas ligand-free PIzA acts as a negative regulator for gip expression. Thus, PIzA of B. burgdorferi with a streamlined c-di-GMP signaling system not only controls multiple targets, as previously envisioned, but has also evolved different modes of action. IMPORTANCE The Lyme disease pathogen, Borrelia burgdorferi, has a simple cyclic dimeric GMP (c-di-GMP) signaling system essential for adaptation of the pathogen to the complicated tick environment. The c-di-GMP effector of B. burgdorferi, PIzA, has been shown to regulate multiple cellular processes, including motility, osmolality sensing, and nutrient utilization. The findings of this study demonstrate that PIzA not only controls multiple targets but also has different functional modalities, allowing it to act as both positive and negative regulator of the glp operon expression. This work highlights how bacteria with a small genome can compensate for the limited regulatory repertoire by increasing the complexity of targets and modes of action in their regulatory proteins. |
资助项目 | National Institute of Allergy and Infectious Diseases[AI083640] ; National Institute of Allergy and Infectious Diseases[AI117198] ; National Science Foundation of China[31270103] ; National Science Foundation of China[31070050] ; National Center for Research Resources, NIH[C06 RR015481-01] |
WOS研究方向 | Microbiology |
语种 | 英语 |
出版者 | AMER SOC MICROBIOLOGY |
WOS记录号 | WOS:000448034900002 |
源URL | [http://202.127.146.157/handle/2RYDP1HH/5872] |
专题 | 中国科学院武汉植物园 |
通讯作者 | Yang, X. Frank |
作者单位 | 1.Univ Calif Davis, Sch Vet Med, Comparat Pathol Lab, Davis, CA 95616 USA 2.Alcami Corp, Durham, NC USA 3.Univ Wyoming, Dept Mol Biol, Laramie, WY 82071 USA 4.Wenzhou Med Univ, Sch Lab Med, Key Lab Lab Med, Minist Educ China, Wenzhou, Zhejiang, Peoples R China 5.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Agr & Environm Microbiol, Environm Microbiol Grp, Wuhan, Hubei, Peoples R China 6.Indiana Univ Sch Med, Dept Microbiol & Immunol, Indianapolis, IN 46202 USA |
推荐引用方式 GB/T 7714 | Yang, X. Frank,Gomelsky, Mark,Carrasco, Sebastian E.,et al. Positive and Negative Regulation of Glycerol Utilization by the c-di-GMP Binding Protein PIzA in Borrelia burgdorferi[J]. JOURNAL OF BACTERIOLOGY,2018,200(22):11. |
APA | Yang, X. Frank.,Gomelsky, Mark.,Carrasco, Sebastian E..,He, Ming.,Troxell, Bryan.,...&Zhang, Jun-Jie.(2018).Positive and Negative Regulation of Glycerol Utilization by the c-di-GMP Binding Protein PIzA in Borrelia burgdorferi.JOURNAL OF BACTERIOLOGY,200(22),11. |
MLA | Yang, X. Frank,et al."Positive and Negative Regulation of Glycerol Utilization by the c-di-GMP Binding Protein PIzA in Borrelia burgdorferi".JOURNAL OF BACTERIOLOGY 200.22(2018):11. |
入库方式: OAI收割
来源:武汉植物园
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