Homologous RNA secondary structure duplications in 3 ' untranslated region influence subgenomic RNA production and replication of dengue virus
文献类型：期刊论文
| 作者 | Yuan, Zhiming2; Xia, Han2; Zhang, Bo2; Zhang, Qiuyan1,2; Liu, Yi2; Liu, Xiaoyun1,2 |
| 刊名 | VIROLOGY
 |
| 出版日期 | 2018-11-01 |
| 卷号 | 524页码:114-126 |
| 关键词 | Dengue virus 3 ' untranslated region RNA secondary structure duplication Subgenomic flavivirus RNA Replication |
| ISSN号 | 0042-6822 |
| DOI | 10.1016/j.virol.2018.08.018 |
| 英文摘要 | Subgenomic flavivirus RNA (sfRNA) is essential for flavivirus-induced cytopathicity. sfRNA-deficient mutant dengue virus (DENV) M8 harboring a deleted domain II in the 3' untranslated region (UTR) has severely decreased cytopathicity and replication efficiency. Here, recovered viruses acquired from M8 by serial passages replicated similarly to the wild type (WT) but produced two types of sfRNA, whereas only one was detected in WT infected cells, resulting in mildly decreased cytopathicity. Sequencing and structure prediction showed that recovered viruses acquired a novel additional partial domain I sequence duplication and formed analogous RNA secondary structures. This was the first experimental evidence verifying that a longer precursor may exist in the 3' UTR of DENV. Additionally, the duplicated structures could be compensated by the homologous structures from other flaviviruses, rather than heterologous viruses, implying that homologous secondary structures or higher tertiary structures played more important roles in sfRNA production and viral replication. |
| 资助项目 | Science and Technology Basic Work Program from the Ministry of Science and Technology of China, China[2013FY113500] ; 135 planning program from Wuhan Institute of Virology, China[WIV-135-PY2] ; Science and Technology Project from Wuhan Science and Technology Bureau, China[2018-201261638501] |
| WOS研究方向 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000446415700012 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/5933]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Yuan, Zhiming; Xia, Han |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Special Pathogens & Biosafety, Wuhan, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yuan, Zhiming,Xia, Han,Zhang, Bo,et al. Homologous RNA secondary structure duplications in 3 ' untranslated region influence subgenomic RNA production and replication of dengue virus[J]. VIROLOGY,2018,524:114-126. |
| APA | Yuan, Zhiming,Xia, Han,Zhang, Bo,Zhang, Qiuyan,Liu, Yi,&Liu, Xiaoyun.(2018).Homologous RNA secondary structure duplications in 3 ' untranslated region influence subgenomic RNA production and replication of dengue virus.VIROLOGY,524,114-126. |
| MLA | Yuan, Zhiming,et al."Homologous RNA secondary structure duplications in 3 ' untranslated region influence subgenomic RNA production and replication of dengue virus".VIROLOGY 524(2018):114-126. |
入库方式： OAI收割
来源：武汉植物园
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