Polarity-Tuning Derivatization-LC-MS Approach for Probing Global Carboxyl-Containing Metabolites in Colorectal Cancer
文献类型：期刊论文
| 作者 | Wu, Jian-Lin5; Liu, Liang5; Hsiao, W. L. Wendy5; Fu, Li3,4; Huang, Guoxin5; Guo, Ming-Quan1; Sun, Baoqing2; Tan, Binbin3,4; Li, Na5; Bian, Xiqing5 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2018-10-02 |
| 卷号 | 90期号:19页码:11210-11215 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.8b01873 |
| 英文摘要 | Carboxyl-containing metabolites (CCMs) widely exist in living systems and are the essential components for life. Global characteristics of CCMs in biological samples are critical for the understanding of physiological processes and the discovery for the onset of relevant diseases. However, their determination represents a challenge due to enormous polarity differences, structural diversity, high structural similarity, and poor ionization efficiency in mass spectrometry. Herein, 5-(chisopropylamino)-amylamine (DIAAA) derivatization coupled with liquid chromatog- raphy-mass spectrometry (LC-MS) was developed for mapping the CCMs. With this methodology, the sensitivity was significantly enhanced. More importantly, the hydrophobicity of polar CCMs, amino acids, TCA cycle intermediates, and short-chain fatty acids and the hydrophilicity of low-polar CCMs, long-chain fatty acids, and bile acids were significantly increased, resulting in a remarkable separation efficiency for which 68 CCMs can be simultaneously determined. Furthermore, the polarity-tuning effect was confirmed to be induced by the different impacts of aliphatic chains and nitrogen atom in DIAAA, the latter existing as a cation in the acidic mobile phase, using different derivatization reagents. Finally, this derivatization method was utilized to hunt for the potential biomarkers in colorectal cancer (CRC) patients and 52 CCMs, related with several key metabolic pathways, including amino acids metabolism, TCA cycle, fatty acid metabolism, pyruvate metabolism, and gut flora metabolism were identified. This innovative polarity-tuning derivatizationLC-MS approach was proved to be a valuable tool for probing global metabolome with high separation efficiency and sensitivity in various biological samples. |
| 资助项目 | Macau Science and Technology Development Fund[009/2017/A1] ; National Natural Science Foundation of China[81772957] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000446542600009 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/5978]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Wu, Jian-Lin; Liu, Liang; Li, Na |
| 作者单位 | 1.Chinese Acad Sci, Sinoafrica Joint Res Ctr, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan Bot Garden, Wuhan 430074, Hubei, Peoples R China 2.Guangzhou Med Univ, State Key Lab Resp Dis, Natl Clin Ctr Resp Dis, Guangzhou Inst Resp Dis,Affiliated Hosp 1, Guangzhou, Guangdong, Peoples R China 3.Shenzhen Univ, Sch Med, Carson Int Canc Res Ctr, Shenzhen 518060, Peoples R China 4.Shenzhen Univ, Sch Med, Guangdong Key Lab Genome Stabil & Dis Prevent, Dept Pharmacol, Shenzhen 518060, Peoples R China 5.Macau Univ Sci & Technol, Macau Inst Appl Res Med & Hlth, State Key Lab Qual Res Chinese Med, Macau, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Jian-Lin,Liu, Liang,Hsiao, W. L. Wendy,et al. Polarity-Tuning Derivatization-LC-MS Approach for Probing Global Carboxyl-Containing Metabolites in Colorectal Cancer[J]. ANALYTICAL CHEMISTRY,2018,90(19):11210-11215. |
| APA | Wu, Jian-Lin.,Liu, Liang.,Hsiao, W. L. Wendy.,Fu, Li.,Huang, Guoxin.,...&Bian, Xiqing.(2018).Polarity-Tuning Derivatization-LC-MS Approach for Probing Global Carboxyl-Containing Metabolites in Colorectal Cancer.ANALYTICAL CHEMISTRY,90(19),11210-11215. |
| MLA | Wu, Jian-Lin,et al."Polarity-Tuning Derivatization-LC-MS Approach for Probing Global Carboxyl-Containing Metabolites in Colorectal Cancer".ANALYTICAL CHEMISTRY 90.19(2018):11210-11215. |
入库方式： OAI收割
来源：武汉植物园
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