Characterization of a New Cyclohexylamine Oxidase From Acinetobacter sp YT-02
文献类型：期刊论文
| 作者 | Yan, Da-zhong2; Gan, Ya-ting2; Ning, Shang-bo2; Chen, Yuan-yuan2; Fang, Ti1; Han, Zheng-gang2; Zhou, Hui2 |
| 刊名 | FRONTIERS IN MICROBIOLOGY
 |
| 出版日期 | 2018-11-22 |
| 卷号 | 9页码:9 |
| ISSN号 | 1664-302X |
| 关键词 | cyclohexylamine oxidase Acinetobacter sp YT-02 cyclohexylamine cyclohexanone biodegradation |
| DOI | 10.3389/fmicb.2018.02848 |
| 英文摘要 | Cyclohexylamine (CHAM) is widely used in various industries, but it is harmful to human beings and the environment. Acinetobacter sp. YT-02 can degrade CHAM via cyclohexanone as an intermediate. In this study, the cyclohexylamine oxidase (CHAO) gene from Acinetobacter sp. YT-02 was cloned. Amino acid sequence alignment indicated that the cyclohexylamine oxidase (CHAO(YT)-02) was 48% identical to its homolog from Brevibacterium oxydans IH-35A (CHAO(IH)_(35)). The enzyme was expressed in Escherichia coil BL21 (DE3), and purified to apparent homogeneity by Ni-affinity chromatography. The purified enzyme was proposed to be a dimer of molecular mass of approximately 91 kDa. The enzyme exhibited its maximum activity at 50 degrees C and at pH 7.0. The enzyme was thermolabile as demonstrated by loss of important percentage of its maximal activity after 30 min incubation at 50 degrees C. Metal ions Mg2+, Co2+, and K+ had certain inhibitory effect on the enzyme activity. The kinetic parameters K-m and V-max were 0.25 +/- 0.02 mM and 4.3 +/- 0.083 mu M min(-1), respectively. The biochemical properties, substrate specificities, and three-dimensional structures of CHAO(YT-02) and CHAO(IH-35) were compared. Our results are helpful to elucidate the mechanism of microbial degradation of CHAM in the strain YT-02. In addition, CHAO(YT-02), as a potential biocatalyst, is promising in controlling CHAM pollution and deracemization of chiral amines. |
| 资助项目 | National Natural Science Foundation of China[31270112] ; National Natural Science Foundation of China[31400114] |
| WOS研究方向 | Microbiology |
| 语种 | 英语 |
| 出版者 | FRONTIERS MEDIA SA |
| WOS记录号 | WOS:000450894600001 |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/6149]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Yan, Da-zhong |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan, Hubei, Peoples R China 2.Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yan, Da-zhong,Gan, Ya-ting,Ning, Shang-bo,et al. Characterization of a New Cyclohexylamine Oxidase From Acinetobacter sp YT-02[J]. FRONTIERS IN MICROBIOLOGY,2018,9:9. |
| APA | Yan, Da-zhong.,Gan, Ya-ting.,Ning, Shang-bo.,Chen, Yuan-yuan.,Fang, Ti.,...&Zhou, Hui.(2018).Characterization of a New Cyclohexylamine Oxidase From Acinetobacter sp YT-02.FRONTIERS IN MICROBIOLOGY,9,9. |
| MLA | Yan, Da-zhong,et al."Characterization of a New Cyclohexylamine Oxidase From Acinetobacter sp YT-02".FRONTIERS IN MICROBIOLOGY 9(2018):9. |
入库方式： OAI收割
来源：武汉植物园
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