热门
Rapid re-evaluation of bioactive saponins from Paris polyphylla using affinity ultrafiltration-LC/MS with multiple drug targets
文献类型：期刊论文
| 作者 | Guo, Mingquan1,2; Chen, Guilin1,2 |
| 刊名 | INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
 |
| 出版日期 | 2018-11-01 |
| 卷号 | 434页码:87-92 |
| ISSN号 | 1387-3806 |
| 关键词 | Ultrafiltration-LC/MS Polyphyllin Topoisomerase I and II Cyclooxygenase 2 Multiple drug targets |
| DOI | 10.1016/j.ijms.2018.09.016 |
| 英文摘要 | Paris polyphylla Smith var. yunnanensis (P. polyphylla) has been widely used as a traditional Chinese medicine for centuries. The Chinese Pharmacopoeia (2015) suggests that the four steroidal saponins, namely polyphyllin I, II, VI and VII, are considered to be the marker components for the quality control (QC) of this herb medicine. However, the active components responsible for the anti-cancer and anti-inflammatory effects of P. polyphylla and the underlying mechanisms of action remain elusive. In the present study, ultrafiltration HPLC-MS (UF-LC-MS) with the multiple drug targets of topoisomerase I, II (Top I, II) and cyclooxygenase 2 (COX-2) was carried out to systematically investigate the mechanisms of action in a multi-component manner. The results revealed that the polyphyllin I displayed not only higher binding affinity to the three drug targets, but also stronger inhibitory activities, as compared with the positive controls. Thus, COX-2 and Top I could be considered as the anti-cancer and anti-inflammatory targets of P. polyphylla. Further molecular docking studies partly revealed the inhibitory mechanism of action associated with the hydrogen bonds between polyphyllin I and the three target enzymes. This study showcased the high efficiency of OF-LC/MS in exploring the potential bioactive components from P. polyphylla and its possible mechanisms of action in a multi-target and multi-component manner. (C) 2018 Elsevier B.V. All rights reserved. |
| 资助项目 | National Natural Science Foundation of China[81673580] ; Major Project for Special Technology Innovation of Hubei Province - Sinoforeign Cooperation Project[2017AHB054] |
| WOS研究方向 | Physics ; Spectroscopy |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCIENCE BV |
| WOS记录号 | WOS:000449709900013 |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/6164]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Guo, Mingquan |
| 作者单位 | 1.Chinese Acad Sci, Sino Africa Joint Res Ctr, Wuhan 430074, Hubei, Peoples R China 2.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Mingquan,Chen, Guilin. Rapid re-evaluation of bioactive saponins from Paris polyphylla using affinity ultrafiltration-LC/MS with multiple drug targets[J]. INTERNATIONAL JOURNAL OF MASS SPECTROMETRY,2018,434:87-92. |
| APA | Guo, Mingquan,&Chen, Guilin.(2018).Rapid re-evaluation of bioactive saponins from Paris polyphylla using affinity ultrafiltration-LC/MS with multiple drug targets.INTERNATIONAL JOURNAL OF MASS SPECTROMETRY,434,87-92. |
| MLA | Guo, Mingquan,et al."Rapid re-evaluation of bioactive saponins from Paris polyphylla using affinity ultrafiltration-LC/MS with multiple drug targets".INTERNATIONAL JOURNAL OF MASS SPECTROMETRY 434(2018):87-92. |
入库方式： OAI收割
来源：武汉植物园
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。