A homogeneous DNA hybridization system by using a new luminescence terbium chelate
文献类型：期刊论文
| 作者 | Sueda, S; Yuan, JL; Matsumoto, K |
| 刊名 | bioconjugate chemistry
 |
| 出版日期 | 2002-03-01 |
| 卷号 | 13期号:2页码:200-205 |
| 英文摘要 | homogeneous dna hybridization assay based on the luminescence resonance energy transfer (lret) from a new luminescence terbium chelate, n,n,n-1,n-1-[2,6-bis(3'-aminomethyl-1'-pyrazolyl)-4-phenylpyridine]tetrakis(acetic acid) (bpta)-tb3+ (lambda(ex) = 325 nm and lambda(em) = 545 nm) to an organic dye, cy3 (a,. = 548 nm and a,. = 565 nm), has been developed. in the system, two dna probes whose sequences are complementary to the two different consecutive sequences of a target dna are used; one of the probes is labeled with the tb3+ chelate at the t-end, and the other is with cy3 at the 5'-end. labeling of the tb3+ chelate is accomplished via the linkage of a biotin-labeled dna probe with the tb3+ chelate-labeled streptavidin. strong sensitized emission of cy3 was observed upon excitation of the tb3+ chelate at 325 run, when the two probe dnas were hybridized with the target dna. the sensitivity of the assay was very high compared with those of the previous homogeneous-format assays using the conventional organic dyes; the detection limit of the present assay is about 30 pm of the target dna strand. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; biochemistry & molecular biology ; chemistry, multidisciplinary ; chemistry, organic |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | fluorescence energy-transfer ; diketonate-europium chelate ; label ; probes |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000174592600007 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/91437]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Waseda Univ, Dept Chem, Shinjuku Ku, Tokyo 1698555, Japan 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Analyt Chem, Dalian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sueda, S,Yuan, JL,Matsumoto, K. A homogeneous DNA hybridization system by using a new luminescence terbium chelate[J]. bioconjugate chemistry,2002,13(2):200-205. |
| APA | Sueda, S,Yuan, JL,&Matsumoto, K.(2002).A homogeneous DNA hybridization system by using a new luminescence terbium chelate.bioconjugate chemistry,13(2),200-205. |
| MLA | Sueda, S,et al."A homogeneous DNA hybridization system by using a new luminescence terbium chelate".bioconjugate chemistry 13.2(2002):200-205. |
入库方式： OAI收割
来源：大连化学物理研究所
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