Modeling of protein adsorption in membrane affinity chromatography
文献类型：期刊论文
| 作者 | Hao, WQ; Chen, ZA; Wang, JD; Liu, XL |
| 刊名 | analytical letters
 |
| 出版日期 | 2004 |
| 卷号 | 37期号:7页码:1319-1338 |
| 关键词 | membrane affinity chromatography ideal adsorption model equilibrium-dispersive model cellulose diacetate/polyethyleneimine blend membranes frontal analysis bovine serum albumin |
| 产权排序 | 1;1 |
| 英文摘要 | for the design of affinity membranes, protein adsorption in membrane affinity chromatography (mac) was studied by frontal analysis. according to fast mass transfer, small thickness of affinity membranes and high affinity between the protein and the ligand, an ideal adsorption (ia) model was proposed for mac and was used together with equilibrium-dispersive (e-d) model to describe the adsorption of bovine serum albumin (bsa) onto cellulose diacetate/polyethyleneimine (ca/pei) blend membranes with and without cu2+ chelating. e-d model was found to better describe the initial region of experimental breakthrough curves. the influence of axial dispersion was revealed and it showed the importance of design of the module to homogenously distribute feed solution. ia model was found to be better for the whole experimental breakthrough curve. according to it, the capacity of affinity membranes and the specificity of the interaction are of equal importance for the design of affinity membranes. an optimum feed concentration was also found in the operation of mac. the discrepancy between experimental optimum feed concentrations and predicted ones from ia model may be due to the ignorance of some experimental effects such as axial dispersion. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | sorption kinetics ; bioseparations ; separation ; breakthrough ; columns ; design |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000221935400005 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/91835]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116012, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hao, WQ,Chen, ZA,Wang, JD,et al. Modeling of protein adsorption in membrane affinity chromatography[J]. analytical letters,2004,37(7):1319-1338. |
| APA | Hao, WQ,Chen, ZA,Wang, JD,&Liu, XL.(2004).Modeling of protein adsorption in membrane affinity chromatography.analytical letters,37(7),1319-1338. |
| MLA | Hao, WQ,et al."Modeling of protein adsorption in membrane affinity chromatography".analytical letters 37.7(2004):1319-1338. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。