Highly sensitive determination of the methylated p16 gene in cancer patients by microchip electrophoresis
文献类型：期刊论文
| 作者 | Zhou, XM; Shao, SJ; Xu, GD; Zhong, RT; Liu, DY; Tang, JW; Gao, YN; Cheng, SJ; Lin, BC |
| 刊名 | journal of chromatography b-analytical technologies in the biomedical and life sciences
 |
| 出版日期 | 2005-02-25 |
| 卷号 | 816期号:1-2页码:145-151 |
| 关键词 | microchip electrophoresis DNA methylation cancer diagnosis |
| ISSN号 | 1570-0232 |
| 产权排序 | 1;1 |
| 通讯作者 | 林炳承 |
| 英文摘要 | the p16 tumor suppressor gene is inactivated by promoter region hypermethylation in many types of tumor. recent studies showed that aberrant methylation of the p16 gene is an early event in many tumors, especially in lung cancer, and may constitute a new biomarker for early detection and monitoring of prevention trials. we detected tumor-associated aberrant hypermethylation of the p16 gene in plasma and tissue dna from 153 specimens using a modified semi-nested methylation-specific pcr (msp) combining plastic microchip electrophoresis or slab gel electrophoresis, respectively. specimens were from 79 lung cancer patients, 15 abdominal tumor patients, 30 positive controls and 30 negative controls. the results showed that the positive rate obtained by microchip electrophoresis was more than 26.6% higher and the same speciticity was kept when compared with slab gel electrophoresis. the microchip electrophoresis can rapidly and accurately analyze the pcr products of methylated dna and obviously improve the positive rate of diagnosis of cancer patients when compared with gel electrophoresis. this method with the high assay sensitivity might be used for detection of methylation of p16 gene and even to facilitate early diagnosis of cancer patients. (c) 2004 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | capillary-electrophoresis ; lung-cancer ; promoter hypermethylation ; dna ; serum ; plasma ; chip |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000226879800019 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/92145]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100000, Peoples R China 3.Dalian Med Univ, Dalian 116023, Peoples R China 4.Peking Union Med Coll, Canc Inst Hosp, Dept Chem Etiol & Carcinogenesis, Beijing 100021, Peoples R China 5.Chinese Acad Med Sci, Beijing 100021, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, XM,Shao, SJ,Xu, GD,et al. Highly sensitive determination of the methylated p16 gene in cancer patients by microchip electrophoresis[J]. journal of chromatography b-analytical technologies in the biomedical and life sciences,2005,816(1-2):145-151. |
| APA | Zhou, XM.,Shao, SJ.,Xu, GD.,Zhong, RT.,Liu, DY.,...&Lin, BC.(2005).Highly sensitive determination of the methylated p16 gene in cancer patients by microchip electrophoresis.journal of chromatography b-analytical technologies in the biomedical and life sciences,816(1-2),145-151. |
| MLA | Zhou, XM,et al."Highly sensitive determination of the methylated p16 gene in cancer patients by microchip electrophoresis".journal of chromatography b-analytical technologies in the biomedical and life sciences 816.1-2(2005):145-151. |
入库方式： OAI收割
来源：大连化学物理研究所
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