Protocol of capillary isoelectric focusing to separate extremely acidic and basic proteins
文献类型：期刊论文
| 作者 | Yang, C; Zhang, WB; Zhang, J; Duan, JC; Zhang, YK |
| 刊名 | journal of separation science
 |
| 出版日期 | 2005 |
| 卷号 | 28期号:1页码:78-86 |
| 关键词 | isoelectric focusing pH gradient extremely acidic and basic proteins |
| ISSN号 | 1615-9306 |
| 产权排序 | 1;1 |
| 通讯作者 | 张玉奎 |
| 英文摘要 | a new set-up was constructed for capillary isoelectric focusing (cief) involving a sampling capillary as a bypass fixed to the separation capillary. sample solutions were subjected to a previously established ph gradient from the sample capillary. besides performing conventional cief, the separation of ampholytic compounds with isoelectric points (p/s) beyond the ph gradient was carried out on this system. this method was termed as ph gradient driven electrophoresis (pgde) and the basic mathematical expressions were derived to express the dynamic fundamentals. proteins such as lysozyme, cytochrome c, and pepsin with p/s higher than 10 or below 3 were separated in a ph gradient provided by pharmalyte (ph 3-10). finally, this protocol convincingly exhibited its potential in the separation of a solution of chicken egg white. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | high-performance electrophoresis ; equilibrium-gradient methods ; zone electrophoresis ; electrokinetic chromatography ; experimental validation ; computer-simulation ; isotachophoresis ; system |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000226463300010 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/92185]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, C,Zhang, WB,Zhang, J,et al. Protocol of capillary isoelectric focusing to separate extremely acidic and basic proteins[J]. journal of separation science,2005,28(1):78-86. |
| APA | Yang, C,Zhang, WB,Zhang, J,Duan, JC,&Zhang, YK.(2005).Protocol of capillary isoelectric focusing to separate extremely acidic and basic proteins.journal of separation science,28(1),78-86. |
| MLA | Yang, C,et al."Protocol of capillary isoelectric focusing to separate extremely acidic and basic proteins".journal of separation science 28.1(2005):78-86. |
入库方式： OAI收割
来源：大连化学物理研究所
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