On-line concentration of proteins in pressurized capillary electrochromatography coupled with electrospray ionization-mass spectrometry
文献类型：期刊论文
| 作者 | Liang, Z; Zhang, LH; Duan, JC; Yan, C; Zhang, WB; Zhang, YK |
| 刊名 | electrophoresis
 |
| 出版日期 | 2005-04-01 |
| 卷号 | 26期号:7-8页码:1398-1405 |
| 关键词 | on-line concentration pressurized capillary electrochromatography-mass spectrometry protein |
| ISSN号 | 0173-0835 |
| 产权排序 | 1;1 |
| 通讯作者 | 张玉奎 |
| 英文摘要 | pressurized capillary electrochromatography (pcec) and electrospray ionization-mass spectrometry (esi-ms) have been hyphenated for protein analysis. taken cytochrome c, lysozyme, and insulin as samples, the limits of detection (lods) for absolute concentrations are 10(-11) mol (signal-to-noise ratio s/n = 3) with relative standard deviations (rsds) of retention time and peak area, respectively, of less than 1.7% and 4.8%. in order to improve the detection sensitivity, on-line concentration by field-enhanced sample-stacking effect and chromatographic zone-sharpening effect has been developed, and parameters affecting separation and detection, such as ph and electrolyte concentration in the mobile phase, separation voltage, as well as enrichment voltage and time, have been studied systematically. under the optimized conditions, the lods of the three proteins could be decreased up to 100-fold. in addition, the feasibility of such techniques has been further demonstrated by the analysis of modified insulins at a concentration of 20 mu g/ml. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | performance liquid-chromatography ; preconcentration ; sensitivity ; phase ; flow ; ms |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000228784000014 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/92235]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China 2.Unimicro Technol Inc, Pleasanton, CA USA |
| 推荐引用方式 GB/T 7714 | Liang, Z,Zhang, LH,Duan, JC,et al. On-line concentration of proteins in pressurized capillary electrochromatography coupled with electrospray ionization-mass spectrometry[J]. electrophoresis,2005,26(7-8):1398-1405. |
| APA | Liang, Z,Zhang, LH,Duan, JC,Yan, C,Zhang, WB,&Zhang, YK.(2005).On-line concentration of proteins in pressurized capillary electrochromatography coupled with electrospray ionization-mass spectrometry.electrophoresis,26(7-8),1398-1405. |
| MLA | Liang, Z,et al."On-line concentration of proteins in pressurized capillary electrochromatography coupled with electrospray ionization-mass spectrometry".electrophoresis 26.7-8(2005):1398-1405. |
入库方式： OAI收割
来源：大连化学物理研究所
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