中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
On-line concentration of proteins in pressurized capillary electrochromatography coupled with electrospray ionization-mass spectrometry

文献类型:期刊论文

作者Liang, Z; Zhang, LH; Duan, JC; Yan, C; Zhang, WB; Zhang, YK
刊名electrophoresis
出版日期2005-04-01
卷号26期号:7-8页码:1398-1405
关键词on-line concentration pressurized capillary electrochromatography-mass spectrometry protein
ISSN号0173-0835
产权排序1;1
通讯作者张玉奎
英文摘要pressurized capillary electrochromatography (pcec) and electrospray ionization-mass spectrometry (esi-ms) have been hyphenated for protein analysis. taken cytochrome c, lysozyme, and insulin as samples, the limits of detection (lods) for absolute concentrations are 10(-11) mol (signal-to-noise ratio s/n = 3) with relative standard deviations (rsds) of retention time and peak area, respectively, of less than 1.7% and 4.8%. in order to improve the detection sensitivity, on-line concentration by field-enhanced sample-stacking effect and chromatographic zone-sharpening effect has been developed, and parameters affecting separation and detection, such as ph and electrolyte concentration in the mobile phase, separation voltage, as well as enrichment voltage and time, have been studied systematically. under the optimized conditions, the lods of the three proteins could be decreased up to 100-fold. in addition, the feasibility of such techniques has been further demonstrated by the analysis of modified insulins at a concentration of 20 mu g/ml.
WOS标题词science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]biochemical research methods ; chemistry, analytical
研究领域[WOS]biochemistry & molecular biology ; chemistry
关键词[WOS]performance liquid-chromatography ; preconcentration ; sensitivity ; phase ; flow ; ms
收录类别SCI
原文出处true
语种英语
WOS记录号WOS:000228784000014
公开日期2010-11-30
源URL[http://159.226.238.44/handle/321008/92235]  
专题大连化学物理研究所_中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China
2.Unimicro Technol Inc, Pleasanton, CA USA
推荐引用方式
GB/T 7714
Liang, Z,Zhang, LH,Duan, JC,et al. On-line concentration of proteins in pressurized capillary electrochromatography coupled with electrospray ionization-mass spectrometry[J]. electrophoresis,2005,26(7-8):1398-1405.
APA Liang, Z,Zhang, LH,Duan, JC,Yan, C,Zhang, WB,&Zhang, YK.(2005).On-line concentration of proteins in pressurized capillary electrochromatography coupled with electrospray ionization-mass spectrometry.electrophoresis,26(7-8),1398-1405.
MLA Liang, Z,et al."On-line concentration of proteins in pressurized capillary electrochromatography coupled with electrospray ionization-mass spectrometry".electrophoresis 26.7-8(2005):1398-1405.

入库方式: OAI收割

来源:大连化学物理研究所

浏览0
下载0
收藏0
其他版本

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。