On-line concentration of peptides and proteins with the hyphenation of polymer monolithic immobilized metal affinity chromatography and capillary electrophoresis
文献类型：期刊论文
| 作者 | Zhang, LY; Zhang, LH; Zhang, WB; Zhang, YK |
| 刊名 | electrophoresis
 |
| 出版日期 | 2005-06-01 |
| 卷号 | 26期号:11页码:2172-2178 |
| 关键词 | capillary electrophoresis Cu(II)-immobilized metal affinity chromatography monolithic column on-line concentration peptide protein |
| ISSN号 | 0173-0835 |
| 产权排序 | 1;1 |
| 通讯作者 | 张玉奎 |
| 英文摘要 | an iminodiacetic acid (ida)-type adsorbent is prepared at the one end of a capillary by covalently bonding ida to the monolithic rods of macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate). cu(ii) is later introduced to the support via the interaction with ida. by this means, polymer monolithic immobilized metal affinity chromatography (imac) materials are prepared. with such a column, imac for on-line concentration and capillary electrophoresis (ce) for the subsequent analysis are hyphenated for the analysis of peptides and proteins. the reproducibility of such a column has been proved good with relative standard deviations (rsds) of dead time of less than 5% for injection-to-injection and 12% for column-to-column (n = 3). through application on the analysis of standard peptides and real protein samples, such a technique has shown promising in proteome study. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | micellar electrokinetic chromatography ; mass-spectrometry ; fluorescence detection ; electrochromatography ; phase ; extraction ; separation ; exchange ; columns |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000229901500013 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/92237]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, LY,Zhang, LH,Zhang, WB,et al. On-line concentration of peptides and proteins with the hyphenation of polymer monolithic immobilized metal affinity chromatography and capillary electrophoresis[J]. electrophoresis,2005,26(11):2172-2178. |
| APA | Zhang, LY,Zhang, LH,Zhang, WB,&Zhang, YK.(2005).On-line concentration of peptides and proteins with the hyphenation of polymer monolithic immobilized metal affinity chromatography and capillary electrophoresis.electrophoresis,26(11),2172-2178. |
| MLA | Zhang, LY,et al."On-line concentration of peptides and proteins with the hyphenation of polymer monolithic immobilized metal affinity chromatography and capillary electrophoresis".electrophoresis 26.11(2005):2172-2178. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。