Rapid authentication of ginseng species using microchip electrophoresis with laser-induced fluorescence detection
文献类型：期刊论文
| 作者 | Qin, JH; Leung, FC; Fung, YS; Zhu, DR; Lin, BC |
| 刊名 | analytical and bioanalytical chemistry
 |
| 出版日期 | 2005-02-01 |
| 卷号 | 381期号:4页码:812-819 |
| 关键词 | ginseng short tandem repeats (STR) microchip electrophoresis molecular authentication |
| ISSN号 | 1618-2642 |
| 产权排序 | 1;1 |
| 通讯作者 | 林炳承 |
| 英文摘要 | ginseng is one of the most expensive chinese herbal medicines and the effectiveness of ginseng depends strongly on its botanical sources and the use of different parts of the plants. in this study, a microchip electrophoresis method coupled with the polymerase chain reaction (pcr)-short tandem repeats (str) technique was developed for rapid authentication of ginseng species. a low viscosity hydroxypropyl methylcellulose (hpmc) solution was used as the sieving matrix for separation of the amplified str fragments. the allele sizing of the amplified pcr products could be detected within 240 s or less. good reproducibility and accuracy of the fragment size were obtained with the relative standard deviation for the allele sizes less than 1.0% (n = 11). at two microsatellite loci (ct 12, ca 33), american ginseng had a different allele pattern on the electropherograms compared with that of the oriental ginseng. moreover, cultivated and wild american ginseng can be distinguished on the basis of allele sizing. this work establishes the feasibility of fast genetic authentication of ginseng species by use of microchip electrophoresis. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | polymerase chain-reaction ; north-american ginseng ; total analysis systems ; capillary-electrophoresis ; tandem repeats ; dna ; identification ; standard ; roots ; pcr |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000227450200003 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/92273]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Univ Hong Kong, Dept Zool, Hong Kong, Hong Kong, Peoples R China 3.Univ Hong Kong, Dept Chem, Hong Kong, Hong Kong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qin, JH,Leung, FC,Fung, YS,et al. Rapid authentication of ginseng species using microchip electrophoresis with laser-induced fluorescence detection[J]. analytical and bioanalytical chemistry,2005,381(4):812-819. |
| APA | Qin, JH,Leung, FC,Fung, YS,Zhu, DR,&Lin, BC.(2005).Rapid authentication of ginseng species using microchip electrophoresis with laser-induced fluorescence detection.analytical and bioanalytical chemistry,381(4),812-819. |
| MLA | Qin, JH,et al."Rapid authentication of ginseng species using microchip electrophoresis with laser-induced fluorescence detection".analytical and bioanalytical chemistry 381.4(2005):812-819. |
入库方式： OAI收割
来源：大连化学物理研究所
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