Simultaneous and ultrarapid determination of reactive oxygen species and reduced glutathione in apoptotic leukemia cells by microchip electrophoresis
文献类型：期刊论文
| 作者 | Qin, JH; Ye, NN; Yu, LF; Liu, DY; Fung, YS; Wang, W; Ma, XJ; Lin, BC |
| 刊名 | electrophoresis
 |
| 出版日期 | 2005-03-01 |
| 卷号 | 26期号:6页码:1155-1162 |
| 关键词 | acute promyelocytic leukemia apoptosis microchip electrophoresis miniaturization reactive oxygen species reduced glutathione |
| ISSN号 | 0173-0835 |
| 产权排序 | 1;1 |
| 通讯作者 | 林炳承 |
| 英文摘要 | a microchip electrophoresis method coupled with laser-induced fluorescence (lif) detection was established for simultaneous determination of two kinds of intracellular signaling molecules (reactive oxygen species, ros, and reduced glutathione, gsh) related to apoptosis and oxidative stress. as the probe dihydrorhodamine-123 (dhr123) can be converted intracellularly by ros to the fluorescent rhodamine-123 (rh123), and the probe naphthalene-2,3-dicarboxaldehyde (nda) can react quickly with gsh to produce a fluorescent adduct, rapid determination of rh-123 and gsh was achieved on a glass microchip within 27 s using a 20 mm borate buffer (ph 9.2). the established method was tested to measure the intracellular ros and gsh levels in acute promyelocytic leukemia (apl)-derived nb4 cells. an elevation of intracellular ros and depletion of gsh were observed in apoptotic n134 cells induced by arsenic trioxide (as(2)o(3)) at low concentration (1-2 mu m). buthionine sulfoximine (bso), in combination with as(2)o(3) enhanced the decrease of reduced gsh to a great extent. the combined treatment of as(2)o(3) and hydrogen peroxide (h2o2) led to an inverse relationship between the concentrations of ros and gsh obtained, showing the proposed method can readily evaluate the generation of ros, which occurs simultaneously with the consumption of the inherent antioxidant. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | capillary-electrophoresis ; arsenic trioxide ; system ; derivatization ; chromatography ; quantitation ; lines |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000228170600015 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/92275]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Univ Hong Kong, Dept Chem, Hong Kong, Hong Kong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qin, JH,Ye, NN,Yu, LF,et al. Simultaneous and ultrarapid determination of reactive oxygen species and reduced glutathione in apoptotic leukemia cells by microchip electrophoresis[J]. electrophoresis,2005,26(6):1155-1162. |
| APA | Qin, JH.,Ye, NN.,Yu, LF.,Liu, DY.,Fung, YS.,...&Lin, BC.(2005).Simultaneous and ultrarapid determination of reactive oxygen species and reduced glutathione in apoptotic leukemia cells by microchip electrophoresis.electrophoresis,26(6),1155-1162. |
| MLA | Qin, JH,et al."Simultaneous and ultrarapid determination of reactive oxygen species and reduced glutathione in apoptotic leukemia cells by microchip electrophoresis".electrophoresis 26.6(2005):1155-1162. |
入库方式： OAI收割
来源：大连化学物理研究所
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