Capillary zone electrophoresis characterization of low molecular weight heparin binding to interleukin 2
文献类型：期刊论文
| 作者 | Liang, AY; He, XY; Du, YG; Wang, KY; Fung, YS; Lin, BC |
| 刊名 | journal of pharmaceutical and biomedical analysis
 |
| 出版日期 | 2005-07-01 |
| 卷号 | 38期号:3页码:408-413 |
| 关键词 | low molecular weight heparin interleukin 2 capillary zone electrophoresis interaction |
| ISSN号 | 0731-7085 |
| 产权排序 | 1;1 |
| 通讯作者 | 林炳承 |
| 英文摘要 | a method based on capillary zone electrophoresis (cze) was used to study the interaction between low molecular weight heparin (lmwh) and interleukin 2 (il-2). the results showed that the increase of the concentration of lmwh led to the decrease of the peak height and the increase of the peak width of il-2, but the peak areas were kept constant. the binding constant of il-2 with lmwh was calculated as 1.2 x 10(6) m(-1) by scatchard analysis, which is in good agreement with the results found in the references using enzyme-linked immunosorbent assay (elisa). the results demonstrated that the interaction between il-2 and lmwh is of fast on-and-off kinetic binding reaction. cze might be used to study not only slow on-and-off rates interactions, but also fast on-and-off rates ones. the binding constant can be calculated easily, and the method can be applied to study a wide range of heparin-protein interactions. (c) 2005 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences ; life sciences & biomedicine |
| 类目[WOS] | chemistry, analytical ; pharmacology & pharmacy |
| 研究领域[WOS] | chemistry ; pharmacology & pharmacy |
| 关键词[WOS] | fibroblast-growth-factor ; human recombinant interleukin-2 ; amyloid-p component ; association constants ; antithrombin-binding ; complex-formation ; protein-binding ; affinity ; domain ; drug |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000230235200004 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/92281]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Chinese Acad Sci, Shanghai Acad Life Sci, Inst Biochem & Cell Biol, Shanghai, Peoples R China 4.Univ Hong Kong, Dept Chem, Hong Kong, Hong Kong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liang, AY,He, XY,Du, YG,et al. Capillary zone electrophoresis characterization of low molecular weight heparin binding to interleukin 2[J]. journal of pharmaceutical and biomedical analysis,2005,38(3):408-413. |
| APA | Liang, AY,He, XY,Du, YG,Wang, KY,Fung, YS,&Lin, BC.(2005).Capillary zone electrophoresis characterization of low molecular weight heparin binding to interleukin 2.journal of pharmaceutical and biomedical analysis,38(3),408-413. |
| MLA | Liang, AY,et al."Capillary zone electrophoresis characterization of low molecular weight heparin binding to interleukin 2".journal of pharmaceutical and biomedical analysis 38.3(2005):408-413. |
入库方式： OAI收割
来源：大连化学物理研究所
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