Instability of anthocyanin accumulation in Vitis vinifera L. var. Gamay Freaux suspension cultures
文献类型：期刊论文
| 作者 | Qu, JG; Zhang, W; Yu, XJ; Jin, MF |
| 刊名 | biotechnology and bioprocess engineering
 |
| 出版日期 | 2005-03-01 |
| 卷号 | 10期号:2页码:155-161 |
| 关键词 | plant cell culture subculture secondary metabolite anthocyanin instability Vitis vinifera |
| ISSN号 | 1226-8372 |
| 产权排序 | 1;1 |
| 通讯作者 | 张卫 |
| 英文摘要 | the inherent instability of metabolite production in plant cell culture-based bioprocessing is a major problem hindering its commercialization. to understand the extent and causes of this instability, this study was aimed at understanding the variability of anthocyanin accumulation during long-term subcultures, as well as within subculture batches, in vitis vinifera cell cultures. therefore, four cell line suspensions of vitis vinitera l. var. gamay freaux, a, b, c and d, originated from the same callus by cell-aggregate cloning, were established with starting anthocyanin contents of 2.73 +/- 0.15, 1.45 +/- 0.04, 0.77 +/- 0.024 and 0.27 +/- 0.04 cv (color value)/g-fcw (fresh cell weight), respectively. during weekly subculturing of 33 batches over 8 months, the anthocyanin biosynthetic capacity was gradually lost at various rates, for all four cell lines, regardless of the significant difference in the starting anthocyanin content. contrary to this general trend, a significant fluctuation in the anthocyanin content was observed, but with an irregular cyclic pattern. the variabilities in the anthocyanin content between the subcultures for the 33 batches, as represented by the variation coefficient (vc), were 58, 57, 54, and 84% for v vinifera cell lines a, b, c and d, respectively. within one subculture, the vcs from 12 replicate flasks for each of 12 independent subcultures were averaged, and found to be 9.7%, ranging from 4 to 17%. high- and low-producing cell lines, vv05 and vv06, with 1.8-fold differences in their basal anthocyanin contents, exhibited different inducibilities to l-phenylalanine feeding, methyl jasmonate and light irradiation. the low-producing cell line, showed greater potential in enhanced the anthocyanin production. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biotechnology & applied microbiology |
| 研究领域[WOS] | biotechnology & applied microbiology |
| 关键词[WOS] | plant-cell cultures ; secondary metabolism ; catharanthus-roseus ; callus |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000228825000009 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/92411]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China 2.Flinders Univ S Australia, Sch Med, CRC Bioprod, Adelaide, SA 5042, Australia 3.Flinders Univ S Australia, Sch Med, Dept Med Biotechnol, Adelaide, SA 5042, Australia |
| 推荐引用方式 GB/T 7714 | Qu, JG,Zhang, W,Yu, XJ,et al. Instability of anthocyanin accumulation in Vitis vinifera L. var. Gamay Freaux suspension cultures[J]. biotechnology and bioprocess engineering,2005,10(2):155-161. |
| APA | Qu, JG,Zhang, W,Yu, XJ,&Jin, MF.(2005).Instability of anthocyanin accumulation in Vitis vinifera L. var. Gamay Freaux suspension cultures.biotechnology and bioprocess engineering,10(2),155-161. |
| MLA | Qu, JG,et al."Instability of anthocyanin accumulation in Vitis vinifera L. var. Gamay Freaux suspension cultures".biotechnology and bioprocess engineering 10.2(2005):155-161. |
入库方式： OAI收割
来源：大连化学物理研究所
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