Purification of human tissue prokallikrein excreted from insect cells by liquid chromatography
文献类型：期刊论文
| 作者 | Yang, Q; Liang, Z; Zhang, LH; Zhang, XD; Zhao, JY; Zhang, WB; Zhang, YK |
| 刊名 | journal of pharmaceutical and biomedical analysis
 |
| 出版日期 | 2005-09-15 |
| 卷号 | 39期号:3-4页码:848-852 |
| 关键词 | kallikrein purification chromatography mass spectrometry |
| 产权排序 | 1;1 |
| 通讯作者 | 张玉奎 |
| 英文摘要 | tissue kallikrein, generally existing in living bodies as prokallikrein, is a serine proteinase that has proven of great significance to treat hypertension, cardiopathy and nephropathy. although the extraction of tissue kallikrein from human urine is the most commonly used method to obtain such a protein, not only the yield is very little, but also the procedure is rather complex. furthermore, the biological safety is uncertain. therefore, the preparation of such a protein by genetic engineering method, including gene expression, cell culture, separation and purification, is very important. in this paper, a new method to obtain purified tissue prokallikrein excreted from insect cells by liquid chromatography has been proposed. in contrast to the previously published papers, the purification procedure is simplified to only three steps with the final yield of 57% and the purity of 95%, which is not only convenient, but also low-cost and suitable for the large-scale preparation of such a protein. the purified protein is further validated as prokallikrein by high performance liquid chromatography-mass spectrometry and amino acid sequencing. (c) 2005 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences ; life sciences & biomedicine |
| 类目[WOS] | chemistry, analytical ; pharmacology & pharmacy |
| 研究领域[WOS] | chemistry ; pharmacology & pharmacy |
| 关键词[WOS] | urinary kallikrein ; expression ; rats |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000232209700074 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/92489]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China 2.Dalian Med Univ, Dept Nephrol, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Q,Liang, Z,Zhang, LH,et al. Purification of human tissue prokallikrein excreted from insect cells by liquid chromatography[J]. journal of pharmaceutical and biomedical analysis,2005,39(3-4):848-852. |
| APA | Yang, Q.,Liang, Z.,Zhang, LH.,Zhang, XD.,Zhao, JY.,...&Zhang, YK.(2005).Purification of human tissue prokallikrein excreted from insect cells by liquid chromatography.journal of pharmaceutical and biomedical analysis,39(3-4),848-852. |
| MLA | Yang, Q,et al."Purification of human tissue prokallikrein excreted from insect cells by liquid chromatography".journal of pharmaceutical and biomedical analysis 39.3-4(2005):848-852. |
入库方式： OAI收割
来源：大连化学物理研究所
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