Study on the interaction between strychnine and bovine serum albumin by capillary electrophoretic frontal analysis
文献类型：期刊论文
| 作者 | Fu, SP; Zhang, XZ; Zhang, F; Xu, Q; Xiao, HB; Liang, XM |
| 刊名 | chinese chemical letters
 |
| 出版日期 | 2005-09-01 |
| 卷号 | 16期号:9页码:1233-1236 |
| 关键词 | strychnine binding constant capillary electrophoretic frontal analysis bovine serum albumin |
| 产权排序 | 1;1 |
| 通讯作者 | 梁鑫淼 |
| 英文摘要 | the protein binding constant, binding sites of the strychnos alkaloid-strychnine and bovine serum albumin (bsa) was determined by capillary electrophoretic frontal analysis (ce-fa) for the first time. the experiment was carried out in a polyacrylamide-coated fused silica capillary (48.4 cmx50 mu m i.d., 38.1 cm effective length) with 20 mmol/l citrate/mes buffer (ph 6.0, ionic strength 0.17). the applied voltage was 12 kv and detection wavelength was set at 257 nm. the plateau height of the peak was employed to determine the unbound concentration of drug in bsa equilibrated sample solution based on the external drug standard in the absence of protein. the present method provides a convenient, accurate technique for the early stage of drug screening. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, multidisciplinary |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | protein-binding |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000231973000027 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/92491]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fu, SP,Zhang, XZ,Zhang, F,et al. Study on the interaction between strychnine and bovine serum albumin by capillary electrophoretic frontal analysis[J]. chinese chemical letters,2005,16(9):1233-1236. |
| APA | Fu, SP,Zhang, XZ,Zhang, F,Xu, Q,Xiao, HB,&Liang, XM.(2005).Study on the interaction between strychnine and bovine serum albumin by capillary electrophoretic frontal analysis.chinese chemical letters,16(9),1233-1236. |
| MLA | Fu, SP,et al."Study on the interaction between strychnine and bovine serum albumin by capillary electrophoretic frontal analysis".chinese chemical letters 16.9(2005):1233-1236. |
入库方式： OAI收割
来源：大连化学物理研究所
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