Analysis of the estrogenic components in kudzu root by bioassay and high performance liquid chromatography
文献类型：期刊论文
| 作者 | Zhang, Y; Chen, JP; Zhang, CZ; Wu, WZ; Liang, XM |
| 刊名 | journal of steroid biochemistry and molecular biology
 |
| 出版日期 | 2005-03-01 |
| 卷号 | 94期号:4页码:375-381 |
| 关键词 | phytoestrogens kudzu root recombinant yeast HPLC |
| 产权排序 | 1;1 |
| 通讯作者 | 梁鑫淼 |
| 英文摘要 | the estrogenic activity of the chinese herb kudzu root was investigated by a recombinant yeast screening assay (yes). isoflavones are the main components in the plant, of which puerarin is the most abundant one. the kudzu root extract was separated into four fractions according to the polarity. the crude extract and its sub-fractions, except the water fraction, showed clear estrogenic activity and the potencies were in the range of 10(-3) to 10(-1) g/l. the ligand potency was used to compare the estrogenic activity of these fractions. the crude extract and its sub-fractions were further analyzed by high performance liquid chromatography (hplc) to correlate the activity and the active components. bioassay and chemical analysis showed that theoretical estrogenic activity expressed as equivalent 17 beta-estradiol concentration or the cumulative effects are comparable to that experimentally determined by yes. the results showed that the high content of isoflavones as well as the high estrogenic activity could make kudzu root extract an interesting candidate for hormone replacement therapy. (c) 2005 elsevier ltd. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemistry & molecular biology ; endocrinology & metabolism |
| 研究领域[WOS] | biochemistry & molecular biology ; endocrinology & metabolism |
| 关键词[WOS] | hormone replacement therapy ; large-scale use ; isoflavones ; flavonoids ; puerariae ; genistein ; daidzein ; extracts ; radix ; risk |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000229445800011 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/92509]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Y,Chen, JP,Zhang, CZ,et al. Analysis of the estrogenic components in kudzu root by bioassay and high performance liquid chromatography[J]. journal of steroid biochemistry and molecular biology,2005,94(4):375-381. |
| APA | Zhang, Y,Chen, JP,Zhang, CZ,Wu, WZ,&Liang, XM.(2005).Analysis of the estrogenic components in kudzu root by bioassay and high performance liquid chromatography.journal of steroid biochemistry and molecular biology,94(4),375-381. |
| MLA | Zhang, Y,et al."Analysis of the estrogenic components in kudzu root by bioassay and high performance liquid chromatography".journal of steroid biochemistry and molecular biology 94.4(2005):375-381. |
入库方式： OAI收割
来源：大连化学物理研究所
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