Screening and analysis of bioactive compounds with biofingerprinting chromatogram analysis of traditional Chinese medicines targeting DNA by microdialysis/HPLC
文献类型：期刊论文
| 作者 | Su, XY; Kong, L; Li, X; Chen, XG; Guo, M; Zou, HF |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2005-05-27 |
| 卷号 | 1076期号:1-2页码:118-126 |
| 关键词 | bioactive compounds biofingerprinting analysis small molecule-DNA binding microdialysis/HPLC traditional Chinese medicines |
| ISSN号 | 0021-9673 |
| 产权排序 | 1;1 |
| 通讯作者 | 邹汉法 |
| 英文摘要 | biofingerprinting chromatogram, analysis, which is defined as the comparison of fingerprinting chromatograms of the extract of traditional chinese medicines (tcms) before and after the interaction with biological systems (dna, protein. cell. etc.), was proposed for screening and analysis of the multiple bioactive compounds in tcms. a method of microdialysis sampling combined with high performance liquid chromatography (hplc) was applied to the study of dna-binding property for the extracts of tcms. seven compounds were found to bind to calf thymus dna (ct-dna) from the tcms of coptis chinensis franch (coptis), but only three ones from phellodendron amurense rupr. (phellodendron) and none from sophoraflavescens ait. (sophora) to bind to ct-dna. respectively. three of them were identified as berberine, palmatine and jatrorrhizine and their association constants (k) to ct-dna were determined by microdialysis/hplc. competitive binding behaviors of them to ct-dna were also investigated. © 2005 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | performance liquid-chromatography ; human serum-albumin ; biologically-active compounds ; protein interactions ; binding-properties ; angelica-sinensis ; topoisomerase-i ; saiboku-to ; ligands ; spectroscopy |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000229551000015 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/92763]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 2.Dalian Univ, Dept Chem & Chem Technol, Dalian 116622, Peoples R China |
| 推荐引用方式 GB/T 7714 | Su, XY,Kong, L,Li, X,et al. Screening and analysis of bioactive compounds with biofingerprinting chromatogram analysis of traditional Chinese medicines targeting DNA by microdialysis/HPLC[J]. journal of chromatography a,2005,1076(1-2):118-126. |
| APA | Su, XY,Kong, L,Li, X,Chen, XG,Guo, M,&Zou, HF.(2005).Screening and analysis of bioactive compounds with biofingerprinting chromatogram analysis of traditional Chinese medicines targeting DNA by microdialysis/HPLC.journal of chromatography a,1076(1-2),118-126. |
| MLA | Su, XY,et al."Screening and analysis of bioactive compounds with biofingerprinting chromatogram analysis of traditional Chinese medicines targeting DNA by microdialysis/HPLC".journal of chromatography a 1076.1-2(2005):118-126. |
入库方式： OAI收割
来源：大连化学物理研究所
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