Improved performance of comprehensive two-dimensional HPLC separation of traditional Chinese medicines by using a silica monolithic column and normalization of peak heights
文献类型：期刊论文
| 作者 | Hu, LH; Chen, XG; Kong, L; Su, XY; Ye, ML; Zou, HF |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2005-10-28 |
| 卷号 | 1092期号:2页码:191-198 |
| 关键词 | silica monolithic column low-abundant components traditional Chinese medicines data treatment liquid chromatography comprehensive two-dimensional separation |
| ISSN号 | 0021-9673 |
| 产权排序 | 1;1 |
| 通讯作者 | 邹汉法 |
| 英文摘要 | performance of comprehensive two-dimensional liquid chromatography system is greatly improved than we reported previously by using a silica monolithic column as for the second dimensional separation. due to the increase of the elution speed on the second dimensional monolithic column, the first dimensional column efficiency and analysis rate can be greatly improved as comparing with conventionally second dimensional column. the developed system was applied to analysis of methanol extraction of two umbelliferae herbs ligusticum chuanxiong hort. and angelica sinensis (oliv.) diels by using cn column as for the first dimensional separation and a silica monolithic ods column for the second dimensional separation, and the obtained three-dimensional chromatograms were treated by normalization of peak heights with the value of the highest peak or setting a certain value using a software written in-house. it was observed that much more peaks for low-abundant components in tcm extract can clearly be detected here than we reported before, due to the large difference for the amount of components in tcms' extract. with the above improvements in separation performance and data treatment, totally about 120 components in methanol extraction of rhizoma chuanxiong and 100 in a. sinensis were separated with uv detection within 130 min. this result meant that both the number of peaks detected increase twice but the analysis time decease twice if comparing with the previously reported result. (c) 2005 published by elsevier b.v. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | phase liquid-chromatography ; capillary-zone-electrophoresis ; mass-spectrometry ; automated instrumentation ; angelica-sinensis ; complex-mixtures ; peptides ; proteins ; chuanxiong ; resolution |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000232510100004 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/92765]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, LH,Chen, XG,Kong, L,et al. Improved performance of comprehensive two-dimensional HPLC separation of traditional Chinese medicines by using a silica monolithic column and normalization of peak heights[J]. journal of chromatography a,2005,1092(2):191-198. |
| APA | Hu, LH,Chen, XG,Kong, L,Su, XY,Ye, ML,&Zou, HF.(2005).Improved performance of comprehensive two-dimensional HPLC separation of traditional Chinese medicines by using a silica monolithic column and normalization of peak heights.journal of chromatography a,1092(2),191-198. |
| MLA | Hu, LH,et al."Improved performance of comprehensive two-dimensional HPLC separation of traditional Chinese medicines by using a silica monolithic column and normalization of peak heights".journal of chromatography a 1092.2(2005):191-198. |
入库方式： OAI收割
来源：大连化学物理研究所
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