Cloning and structure analysis of hydrogenase gene from Chlamydomonas reinhardtii SE
文献类型：期刊论文
| 作者 | Zhang, LH; Zhang, W; Jin, MF; Zhang, HY; Wang, YJ |
| 刊名 | process biochemistry
 |
| 出版日期 | 2005-09-01 |
| 卷号 | 40期号:9页码:2968-2972 |
| 关键词 | C. reinhardtii SE hydrogenase gene sequence analysis |
| 产权排序 | 1;1 |
| 通讯作者 | yunjiwang |
| 英文摘要 | the cdna of chlamydomonas reinhardtii se encoding hydrogenase (hyda2) was obtained from the total rna of c reinhardtii se by rt-pcr. the dna of hydrogenase was amplified by pcr from the genomic dna of c reinhardtii se. the cdna and dna of hydrogenase were sequenced, respectively. the structure of hydrogenase gene was analyzed by biology software. the open reading frame predicts that the hydrogenase is composed of 3584 bp encoding 505 amino acids in length with a predicted m.w. of 53.69 kda. ten exons (including 1518 bp) and nine introns (including 2066 bp) have been found in the hydrogenase, and there were two potential n-glycosylate sites, eight protein kinase c phosphorylation site, eight casein kinase h phosphorylation site and one sulphorylation in the sequence. the theory pi was 6.15. total number of negatively charged residues (asp + glu) and positively charged residues (arg + lys) were 55 and 61, respectively. (c) 2005 elsevier ltd. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; technology |
| 类目[WOS] | biochemistry & molecular biology ; biotechnology & applied microbiology ; engineering, chemical |
| 研究领域[WOS] | biochemistry & molecular biology ; biotechnology & applied microbiology ; engineering |
| 关键词[WOS] | green-alga ; energy |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000231841800006 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/92783]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Dalian Inst Light Ind, Coll Bio & Food Technol, Dalian 116034, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, LH,Zhang, W,Jin, MF,et al. Cloning and structure analysis of hydrogenase gene from Chlamydomonas reinhardtii SE[J]. process biochemistry,2005,40(9):2968-2972. |
| APA | Zhang, LH,Zhang, W,Jin, MF,Zhang, HY,&Wang, YJ.(2005).Cloning and structure analysis of hydrogenase gene from Chlamydomonas reinhardtii SE.process biochemistry,40(9),2968-2972. |
| MLA | Zhang, LH,et al."Cloning and structure analysis of hydrogenase gene from Chlamydomonas reinhardtii SE".process biochemistry 40.9(2005):2968-2972. |
入库方式： OAI收割
来源：大连化学物理研究所
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