Purification and characterization of recombinant truncated human interleukin-11 expressed as fusion protein in Escherichia coli
文献类型：期刊论文
| 作者 | Tan, HD; Dan, GP; Gong, HY; Cao, LJ |
| 刊名 | biotechnology letters
 |
| 出版日期 | 2005-07-01 |
| 卷号 | 27期号:13页码:905-910 |
| 关键词 | affinity chromatography GST-fusion protein human interleukin-11 |
| ISSN号 | 0141-5492 (paper) 1573-6776 (online) |
| 产权排序 | 1;1 |
| 通讯作者 | 谭海东 |
| 英文摘要 | mature human interleukin-11 (huil-11) is a cytokine consisting of 178 amino acid residues that results from scission of the n-terminal signal peptide, consisting of 21 amino acid residaues, from the corresponding nascent polypeptide. a dna fragment encoding a truncated huil-11 (trhuil-11), with an additional 5 amino acid residues removed from the n-terminus, was cloned into vector pgex-2t between the bamhi site and the ecori site. upon transformation with escherichia coli bl21, the construct over-produced a glutathione s-transferase (gst)-fused protein in a soluble form after iptg induction. the fusion protein was initially fractionated with butyl-sepharose 4 fast flow column and by affinity chromatography using a gsh-sepharose 4b column. on-site enzymatic release with thrombin gave the target protein at 96% purity as judged by sds-page and hplc. expression of the interleukin as a gst-fused protein thus greatly improved downstream processing. subsequent biological activity assay suggested that trhuil-11 had similar activity profile to the naturally produced sample and may be a promising candidate for further development as biopharmaceutical. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biotechnology & applied microbiology |
| 研究领域[WOS] | biotechnology & applied microbiology |
| 关键词[WOS] | rheumatoid-arthritis ; interleukin-11 ; cells |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000231164000004 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/93187]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Duotai Biopharmaceut Co Ltd, Chongqing 200400, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tan, HD,Dan, GP,Gong, HY,et al. Purification and characterization of recombinant truncated human interleukin-11 expressed as fusion protein in Escherichia coli[J]. biotechnology letters,2005,27(13):905-910. |
| APA | Tan, HD,Dan, GP,Gong, HY,&Cao, LJ.(2005).Purification and characterization of recombinant truncated human interleukin-11 expressed as fusion protein in Escherichia coli.biotechnology letters,27(13),905-910. |
| MLA | Tan, HD,et al."Purification and characterization of recombinant truncated human interleukin-11 expressed as fusion protein in Escherichia coli".biotechnology letters 27.13(2005):905-910. |
入库方式： OAI收割
来源：大连化学物理研究所
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