Integration of ion-exchange chromatography fractionation with reversed-phase liquid chromatography-atmospheric pressure chemical ionization mass spectrometer and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for isolation and identification of compounds in Psoralea corylifolia
文献类型：期刊论文
| 作者 | Chen, XG; Kong, L; Su, XY; Pan, CS; Ye, ML; Zou, HF |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2005-09-30 |
| 卷号 | 1089期号:1-2页码:87-100 |
| 关键词 | Psoralea corylifolia ion-exchange chromatography fractionation LC-APCI/MS MALDI-TOF/MS integrated approach |
| ISSN号 | 0021-9673 |
| 产权排序 | 1;1 |
| 通讯作者 | 邹汉法 |
| 英文摘要 | an approach for the separation and identification of components in a traditional chinese medicine psoralea corylifolia was developed. ion-exchange chromatography (iec) was applied for the fractionation of p corylifolia extract, and then followed by concentration of all the fractions with rotary vacuum evaporator. each of the enriched fractions was then further separated on an ods column with detection of uv absorbance and atmospheric pressure chemical ionization mass spectrometer (apci/ms), respectively, and also analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof/ms) with matrix of oxidized carbon nanotubes. totally more than 188 components in p. corylifolia extract were detected with this integrated approach, and 12 of them were preliminary identified according to their uv spectra and mass spectra performed by apci/ms and maldi-tof/ms. the obtained analytical results not only demonstrated the powerful resolution of integration iec fractionation with reversed-phase liquid chromatography (rplc)-apci/ms and maldi-tof/ms for analysis of compounds in a complex sample, but also exhibited the superiority of apci/ms and maldi-tof/ms for identification of low-mass compounds, such as for study of traditional chinese medicines (tcms) and metabolome. (c) 2005 published by elsevier b.v. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | counter-current chromatography ; traditional chinese medicines ; active constituents ; carbon nanotubes ; resolution ; separation ; proteome ; molecules ; ms ; lc |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000231200700012 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/93373]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, XG,Kong, L,Su, XY,et al. Integration of ion-exchange chromatography fractionation with reversed-phase liquid chromatography-atmospheric pressure chemical ionization mass spectrometer and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for isolation and identification of compounds in Psoralea corylifolia[J]. journal of chromatography a,2005,1089(1-2):87-100. |
| APA | Chen, XG,Kong, L,Su, XY,Pan, CS,Ye, ML,&Zou, HF.(2005).Integration of ion-exchange chromatography fractionation with reversed-phase liquid chromatography-atmospheric pressure chemical ionization mass spectrometer and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for isolation and identification of compounds in Psoralea corylifolia.journal of chromatography a,1089(1-2),87-100. |
| MLA | Chen, XG,et al."Integration of ion-exchange chromatography fractionation with reversed-phase liquid chromatography-atmospheric pressure chemical ionization mass spectrometer and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for isolation and identification of compounds in Psoralea corylifolia".journal of chromatography a 1089.1-2(2005):87-100. |
入库方式： OAI收割
来源：大连化学物理研究所
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