Separation and detection of compounds in Honeysuckle by integration of ion-exchange chromatography fractionation with reversed-phase liquid chromatography-atmospheric pressure chemical ionization mass spectrometer and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis
文献类型：期刊论文
| 作者 | Chen, XG; Hu, LH; Su, XY; Kong, L; Ye, ML; Zou, HF |
| 刊名 | journal of pharmaceutical and biomedical analysis
 |
| 出版日期 | 2006-02-24 |
| 卷号 | 40期号:3页码:559-570 |
| 关键词 | Honeysuckle ion-exchange chromatography fractionation LC-APCI/MS MALDI-TOF/MS integrated approach |
| 产权排序 | 1;1 |
| 通讯作者 | 邹汉法 |
| 英文摘要 | a hyphenated method for the isolation and identification of components in a traditional chinese medicine of honeysuckle was developed. ion-exchange chromatography (iec) was chosen for the fractionation of honeysuckle extract, and then followed by concentration of all the fractions with rotary vacuum evaporator. each of the enriched fractions was then further analyzed by reversed-phase liquid chromatography-atmospheric pressure chemical ionization mass spectrometer (rplc-apci/ms) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof/ms) with matrix of oxidized carbon nanotubes, respectively. it can be noted totally more than 117 components were detected by uv detector, apci/ms and maldi-tof/ms in honeysuckle extract except the, 145 components identified by maldi-tof/ms alone with this integrated approach, and 7 of them were preliminary identified according to their uv spectra and mass spectra performed by apci/ms and maldi-tof/ms, respectively. the obtained analytical results not only indicated the approach of integration iec fractionation with rplc-apci/ms and maldi-tof/ms is capable of analyzing complex samples, but also exhibited the potential power of the mass spectrometer in detection of low-mass compounds, such as traditional chinese medicines (tcms) and complex biological samples. (c) 2005 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences ; life sciences & biomedicine |
| 类目[WOS] | chemistry, analytical ; pharmacology & pharmacy |
| 研究领域[WOS] | chemistry ; pharmacology & pharmacy |
| 关键词[WOS] | counter-current chromatography ; traditional chinese medicines ; artemisia-capillaris ; active constituents ; lonicera-japonica ; carbon nanotubes ; chlorogenic acid ; flos-lonicerae ; identification ; resolution |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000235821100010 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/93379]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatograph R&A Ctr, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, XG,Hu, LH,Su, XY,et al. Separation and detection of compounds in Honeysuckle by integration of ion-exchange chromatography fractionation with reversed-phase liquid chromatography-atmospheric pressure chemical ionization mass spectrometer and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis[J]. journal of pharmaceutical and biomedical analysis,2006,40(3):559-570. |
| APA | Chen, XG,Hu, LH,Su, XY,Kong, L,Ye, ML,&Zou, HF.(2006).Separation and detection of compounds in Honeysuckle by integration of ion-exchange chromatography fractionation with reversed-phase liquid chromatography-atmospheric pressure chemical ionization mass spectrometer and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis.journal of pharmaceutical and biomedical analysis,40(3),559-570. |
| MLA | Chen, XG,et al."Separation and detection of compounds in Honeysuckle by integration of ion-exchange chromatography fractionation with reversed-phase liquid chromatography-atmospheric pressure chemical ionization mass spectrometer and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis".journal of pharmaceutical and biomedical analysis 40.3(2006):559-570. |
入库方式： OAI收割
来源：大连化学物理研究所
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