High-performance liquid chromatography-tandem mass spectrometry for identification of isoflavones and description of the biotransformation of kudzu root
文献类型：期刊论文
| 作者 | Zhang, Y; Xu, Q; Zhang, XZ; Chen, JP; Liang, XM; Kettrup, A |
| 刊名 | analytical and bioanalytical chemistry
 |
| 出版日期 | 2005-11-01 |
| 卷号 | 383期号:5页码:787-796 |
| 关键词 | kudzu root isoflavones LC-MS crude beta-glycosidase biotransformation |
| 产权排序 | 1;1 |
| 通讯作者 | 梁鑫淼 |
| 英文摘要 | high-performance liquid chromatography-tandem mass spectrometry has been used to identify isoflavone aglycones and glycosides in kudzu root. fourteen isoflavones were detected. among these, six were identified by comparison with authentic standards. tentative identifications of the other isoflavones are based on uv spectra, mass spectra of protonated and deprotonated molecules, and ms-ms data. several are reported for the first time in kudzu root. the bioactivity and bioavailability of isoflavone aglycones are usually greater than those of their glycosides. to improve the bioavailability of kudzu root isoflavones, crude beta-glycosidases prepared from microbes were used to hydrolyze the isoflavone glycosides. several ms modes are combined not only to identify the isoflavones in kudzu root, but also to describe the biotransformation of kudzu root isoflavone glycosides. it is also proved that crude beta-glycosidases have high selectivity toward the o-glycosides of isoflavones. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | pueraria-lobata ; phenolic glucosides ; red-clover ; phytoestrogens ; soy ; quantitation ; ultraviolet ; separation ; aglycones ; daidzein |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000233874900014 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/93473]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Tech Univ Munich, D-85354 Freising Weihenstephan, Germany 3.GSF Forschungszentrum Umwelt & Gesundheit GMBH, D-85764 Neuherberg, Germany |
| 推荐引用方式 GB/T 7714 | Zhang, Y,Xu, Q,Zhang, XZ,et al. High-performance liquid chromatography-tandem mass spectrometry for identification of isoflavones and description of the biotransformation of kudzu root[J]. analytical and bioanalytical chemistry,2005,383(5):787-796. |
| APA | Zhang, Y,Xu, Q,Zhang, XZ,Chen, JP,Liang, XM,&Kettrup, A.(2005).High-performance liquid chromatography-tandem mass spectrometry for identification of isoflavones and description of the biotransformation of kudzu root.analytical and bioanalytical chemistry,383(5),787-796. |
| MLA | Zhang, Y,et al."High-performance liquid chromatography-tandem mass spectrometry for identification of isoflavones and description of the biotransformation of kudzu root".analytical and bioanalytical chemistry 383.5(2005):787-796. |
入库方式： OAI收割
来源：大连化学物理研究所
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