Rapid protein identification using monolithic enzymatic microreactor and LC-ESI-MS/MS
文献类型：期刊论文
| 作者 | Duan, JC; Liang, Z; Yang, C; Zhang, J; Zhang, LH; Zhang, WB; Zhang, YK |
| 刊名 | proteomics
 |
| 出版日期 | 2006 |
| 卷号 | 6期号:2页码:412-419 |
| 关键词 | enzymatic reactor LC-MS/MS monolithic column protein identification |
| 产权排序 | 1;1 |
| 通讯作者 | 张玉奎 |
| 英文摘要 | a monolithic enzymatic microreactor was prepared in a fused-silica capillary by in situ polymerization of acrylamide, glycidyl methacrylate (gma) and ethylene dimethacrylate (edma) in the presence of a binary porogenic mixture of dodecanol and cyclohexanol, followed by ammonia solution treatment, glutaraldehyde activation and trypsin modification. the choice of acrylamide as co-monomer was found useful to improve the efficiency of trypsin modification, thus, to increase the enzyme activity. the optimized microreactor offered very low back pressure, enabling the fast digestion of proteins flowing through the reactor. the performance of the monolithic microreactor was demonstrated with the digestion of cytochrome c at high flow rate. the digests were then characterized by ce and hplc-ms/ms with the sequence coverage of 57.7%. the digestion efficiency was found over 230 times as high as that of the conventional method. in addition, for the first time, protein digestion carried out in a mixture of water and acn was compared with the conventional aqueous reaction using ms/ms detection, and the former solution was found more compatible and more efficient for protein digestion. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemical research methods ; biochemistry & molecular biology |
| 研究领域[WOS] | biochemistry & molecular biology |
| 关键词[WOS] | mass-spectrometry ; capillary-electrophoresis ; affinity-chromatography ; proteolytic digestion ; trypsin microreactor ; peptide separation ; ion-exchange ; sol-gel ; performance ; chip |
| 收录类别 | ISTP ; SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000235207700003 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/93527]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Duan, JC,Liang, Z,Yang, C,et al. Rapid protein identification using monolithic enzymatic microreactor and LC-ESI-MS/MS[J]. proteomics,2006,6(2):412-419. |
| APA | Duan, JC.,Liang, Z.,Yang, C.,Zhang, J.,Zhang, LH.,...&Zhang, YK.(2006).Rapid protein identification using monolithic enzymatic microreactor and LC-ESI-MS/MS.proteomics,6(2),412-419. |
| MLA | Duan, JC,et al."Rapid protein identification using monolithic enzymatic microreactor and LC-ESI-MS/MS".proteomics 6.2(2006):412-419. |
入库方式： OAI收割
来源：大连化学物理研究所
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