A normal mucin-binding lectin from the sponge Craniella australiensis
文献类型：期刊论文
| 作者 | Xiong, CN; Li, W; Liu, H; Zhang, W; Dou, JL; Bai, XF; Du, YG; Ma, XJ |
| 刊名 | comparative biochemistry and physiology c-toxicology & pharmacology
 |
| 出版日期 | 2006-05-01 |
| 卷号 | 143期号:1页码:9-16 |
| 关键词 | sponge Craniella australiensis mucin-binding lectin glycoproteins mitogenic |
| 产权排序 | 1;1 |
| 通讯作者 | 李伟 |
| 英文摘要 | a lectin, craniella australiensis (cal), was isolated from sponge c. australiensis by ion-exchange on deae-sephacel and purified by gel filtration on sephadex g-150 and hplc on deaf-5pw. the purified lectin was a trimeric protein as revealed by sds-page and maldi-tof analysis. sds-page showed that the cal protein had a molecular mass of 54 kda, and consisted of three 18 kda subunits. gel filtration of purified lectin on sephadex g-200 indicates that it exists as a 54 kda protein in its native state. the amino acid composition was rich in thr and glx. cal was found to agglutinate native and trypsinized human a, b erythrocytes, and agglutinate native erythrocytes of mouse, sheep, rabbit and chicken, and trypsinized erythrocytes of sheep and rabbit. the hemagglutination activity was inhibited by glycoproteins such as psm and asialo-psm, but not by any of the monosaccharides tested. the activity was stable between 20 and 70 c. significant cal activity was observed between ph 5 and 8. the lectin reaction is independent of the presence of divalent cations ca2+ and mg2+. the sequence of n-terminal residues of cal was determined as tsscqsivve. the lectin showed a potent mitogenic response towards balb/c splenocytes. (c) 2005 elsevier inc. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemistry & molecular biology ; endocrinology & metabolism ; toxicology ; zoology |
| 研究领域[WOS] | biochemistry & molecular biology ; endocrinology & metabolism ; toxicology ; zoology |
| 关键词[WOS] | ascidian didemnum-ternatanum ; axinella-polypoides schmidt ; amino-acid-sequence ; geodia-cydonium ; human-lymphocytes ; self recognition ; horseshoe-crab ; purification ; molecules ; mitogen |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000237030600002 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/93659]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Dalian Fisheries Univ, Key & Open Lab Aquat Prod Proc & Utilizat Liaonin, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xiong, CN,Li, W,Liu, H,et al. A normal mucin-binding lectin from the sponge Craniella australiensis[J]. comparative biochemistry and physiology c-toxicology & pharmacology,2006,143(1):9-16. |
| APA | Xiong, CN.,Li, W.,Liu, H.,Zhang, W.,Dou, JL.,...&Ma, XJ.(2006).A normal mucin-binding lectin from the sponge Craniella australiensis.comparative biochemistry and physiology c-toxicology & pharmacology,143(1),9-16. |
| MLA | Xiong, CN,et al."A normal mucin-binding lectin from the sponge Craniella australiensis".comparative biochemistry and physiology c-toxicology & pharmacology 143.1(2006):9-16. |
入库方式： OAI收割
来源：大连化学物理研究所
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