Comprehensive two-dimensional HPLC to study the interaction of multiple components in Rheum palmatum L. with HSA by coupling a silica-bonded HSA column to a silica monolithic ODS column
文献类型：期刊论文
| 作者 | Hu, LH; Li, X; Feng, S; Kong, L; Su, XG; Chen, XG; Oln, F; Ye, ML; Zou, HF |
| 刊名 | journal of separation science
 |
| 出版日期 | 2006-04-01 |
| 卷号 | 29期号:6页码:881-888 |
| 关键词 | comprehensive two-dimensional separation Rheum palmatum L silica-bonded HSA column silica monolithic ODS column |
| ISSN号 | 1615-9306 |
| 产权排序 | 1;1 |
| 通讯作者 | 邹汉法 |
| 英文摘要 | a mode of comprehensive 2-d lc was developed by coupling a silica-bonded hsa column to a silica monolithic ods column. this system combined the affinity property of the hsa column and the high-speed separation ability of the monolithic ods column. the affinity chromatography with hsa-immobilized stationary phase was applied to study the interaction of multiple components in traditional chinese medicines (tcms) with hsa according to their affinity to protein in the first dimension. then the unresolved components retained on the hsa column were further separated on the silica monolithic ods column in the second dimension. by hyphenating the 2-d separation system to diode array detector and ms detectors, the uv and molecular weight information of the separated compounds can also be obtained. the developed separation system was applied to analysis of the extract of rheum palmatum l., a number of low-abundant components can be separated on a single peak from the hsa column after normalization of peak heights. six compounds were preliminarily identified according to their uv and ms spectra. it showed that this system was very useful for biological fingerprinting analysis of the components in tcms and natural products. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | performance liquid-chromatography ; traditional chinese medicines ; human serum-albumin ; affinity-chromatography ; bioactive compounds ; rhizoma-chuanxiong ; angelica-sinensis ; mass-spectrometry ; separation ; phase |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000237162300017 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/93665]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, LH,Li, X,Feng, S,et al. Comprehensive two-dimensional HPLC to study the interaction of multiple components in Rheum palmatum L. with HSA by coupling a silica-bonded HSA column to a silica monolithic ODS column[J]. journal of separation science,2006,29(6):881-888. |
| APA | Hu, LH.,Li, X.,Feng, S.,Kong, L.,Su, XG.,...&Zou, HF.(2006).Comprehensive two-dimensional HPLC to study the interaction of multiple components in Rheum palmatum L. with HSA by coupling a silica-bonded HSA column to a silica monolithic ODS column.journal of separation science,29(6),881-888. |
| MLA | Hu, LH,et al."Comprehensive two-dimensional HPLC to study the interaction of multiple components in Rheum palmatum L. with HSA by coupling a silica-bonded HSA column to a silica monolithic ODS column".journal of separation science 29.6(2006):881-888. |
入库方式： OAI收割
来源：大连化学物理研究所
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