Determination of DL-tetrahydropalmatine in Corydalis yanhusuo by L-tetrahydropalmatine imprinted monolithic column coupling with reversed-phase high performance liquid chromatography
文献类型：期刊论文
| 作者 | Ou, JJ; Kong, L; Pan, CS; Su, XY; Lei, XY; Zou, HF |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2006-06-09 |
| 卷号 | 1117期号:2页码:163-169 |
| 关键词 | molecularly imprinted polymer monolithic column tetrahydropalmatine chiral separation on-line solid-phase extraction Corydalis yanhusuo |
| ISSN号 | 0021-9673 |
| 产权排序 | 1;1 |
| 通讯作者 | 邹汉法 |
| 英文摘要 | a method for direct determination of dl-tetrahydropalmatine (dl-thp) in corydalis yanhusuo, a traditional chinese herb, by l-thp imprinted monolithic precolumn on-line/off-line coupling with reversed-phase high performance liquid chromatography (rp-hplc) was developed. the l-thp imprinted monolithic column has been prepared by in situ polymerization using methacrylic acid (maa) and ethylene dimethacrylate (edma) as functional monomer and cross-linker, respectively. with the optimization of chromatographic conditions, such as mobile phase composition, flow rate, column temperature and sample loading, for the separation of enantiomer, dl-thp was base-line separated on the mip. the imprinted monolithic column was used as a precolumn for fractionation of the c. yanhusuo extract. both the non-retained and retained fractions were separated by rp-hplc. meanwhile, the d-thp and l-thp can be detected in the non-retained and retained fractions, respectively. additionally, direct determination of l-thp using molecularly imprinted monolith on-line coupling with a reversed-phase column was acquired. (c) 2006 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | growth-factor receptor ; environmental water ; mass-spectrometry ; stationary phases ; organic modifier ; extraction ; polymers ; separation ; samples ; enantiomers |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000238005800007 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/93729]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ou, JJ,Kong, L,Pan, CS,et al. Determination of DL-tetrahydropalmatine in Corydalis yanhusuo by L-tetrahydropalmatine imprinted monolithic column coupling with reversed-phase high performance liquid chromatography[J]. journal of chromatography a,2006,1117(2):163-169. |
| APA | Ou, JJ,Kong, L,Pan, CS,Su, XY,Lei, XY,&Zou, HF.(2006).Determination of DL-tetrahydropalmatine in Corydalis yanhusuo by L-tetrahydropalmatine imprinted monolithic column coupling with reversed-phase high performance liquid chromatography.journal of chromatography a,1117(2),163-169. |
| MLA | Ou, JJ,et al."Determination of DL-tetrahydropalmatine in Corydalis yanhusuo by L-tetrahydropalmatine imprinted monolithic column coupling with reversed-phase high performance liquid chromatography".journal of chromatography a 1117.2(2006):163-169. |
入库方式： OAI收割
来源：大连化学物理研究所
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