Analysis of multiplex PCR fragments with PMMA microchip
文献类型：期刊论文
| 作者 | Liu, DY; Zhou, XM; Zhong, RT; Ye, NN; Chang, GH; Xiong, W; Mei, XD; Lin, BC |
| 刊名 | talanta
 |
| 出版日期 | 2006-01-15 |
| 卷号 | 68期号:3页码:616-622 |
| 关键词 | microchip electrophoresis microchip fabrication multiplex PCR |
| 产权排序 | 1;1 |
| 通讯作者 | 林炳承 |
| 英文摘要 | microchip electrophoresis is a promising technique for analysis of bio-molecules. it has the advantages of fast analysis, high sensitivity, high resolution and low-cost of samples. plastic chip has the potential of mass production for clinical use for its advantages in biocompatibility and low cost. in this work, the method for fabrication of poly(methyl methacrylate) (pmma) chip was described, and conditions for dna separation were investigated with the chip. the pmma microchip was used for detection of multiplex pcr products of 18 and 36 cases with sars and hepatitis b virus infection under optimized separation conditions. microchip electrophoresis showed higher sensitivity, higher resolution and less time consumption when compared with gel electrophoresis. the microchip electrophoresis with pmma chip provided a rapid, sensitive and reliable method for analysis of multiplex pcr products. (c) 2005 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | capillary-electrophoresis ; microfluidic chip ; fabrication ; devices ; dna ; molecules ; system |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000234955400018 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/93871]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China 2.Guangzhou First Municipal Peoples Hosp, Guangzhou Med Coll, Dept Clin Lab, Guangzhou 510500, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100000, Peoples R China 4.Chinese Acad Mil Med Sci, Inst Microbiol & Epidemiol, Beijing 100071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, DY,Zhou, XM,Zhong, RT,et al. Analysis of multiplex PCR fragments with PMMA microchip[J]. talanta,2006,68(3):616-622. |
| APA | Liu, DY.,Zhou, XM.,Zhong, RT.,Ye, NN.,Chang, GH.,...&Lin, BC.(2006).Analysis of multiplex PCR fragments with PMMA microchip.talanta,68(3),616-622. |
| MLA | Liu, DY,et al."Analysis of multiplex PCR fragments with PMMA microchip".talanta 68.3(2006):616-622. |
入库方式： OAI收割
来源：大连化学物理研究所
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