Optimization of the cell seeding density and modeling of cell growth and metabolism using the modified Gompertz model for microencapsulated animal cell culture
文献类型：期刊论文
| 作者 | Qi, WT; Zhang, Y; Ma, JA; Guo, X; Xie, YB; Wang, W; Ma, XJ |
| 刊名 | biotechnology and bioengineering
 |
| 出版日期 | 2006-04-05 |
| 卷号 | 93期号:5页码:887-895 |
| 关键词 | microencapsulation microcapsule cell seeding density modeling cell culture |
| 产权排序 | 1;1 |
| 通讯作者 | 马小军 |
| 英文摘要 | cell microericapsulation is one of the promising strategies for the in vitro production of proteins or in vivo delivery of therapeutic products. in order to design and fabricate the optimized microencapsulated cell system, the gompertz model was applied and modified to describe the growth and metabolism of microencapsulated cell, including substrate consumption and product formation. the gompertz model successfully described the cell growth kinetics and the modified gompertz models fitted the substrate consumption and product formation well. it was demonstrated that the optimal initial cell seeding density was about 4-5 x 10(6) cells/ml of microcapsule, in terms of the maximum specific growth rate, the glucose consumption potential and the product formation potential calculated by the gompertz and modified gompertz models. modeling of cell growth and metabolism in microcapsules provides a guideline for optimizing the culture of microencapsulated cells. (c) 2005 wiley periodicals, inc. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biotechnology & applied microbiology |
| 研究领域[WOS] | biotechnology & applied microbiology |
| 关键词[WOS] | mammalian-cells ; cancer growth ; lactate |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000236592000007 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/97129]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qi, WT,Zhang, Y,Ma, JA,et al. Optimization of the cell seeding density and modeling of cell growth and metabolism using the modified Gompertz model for microencapsulated animal cell culture[J]. biotechnology and bioengineering,2006,93(5):887-895. |
| APA | Qi, WT.,Zhang, Y.,Ma, JA.,Guo, X.,Xie, YB.,...&Ma, XJ.(2006).Optimization of the cell seeding density and modeling of cell growth and metabolism using the modified Gompertz model for microencapsulated animal cell culture.biotechnology and bioengineering,93(5),887-895. |
| MLA | Qi, WT,et al."Optimization of the cell seeding density and modeling of cell growth and metabolism using the modified Gompertz model for microencapsulated animal cell culture".biotechnology and bioengineering 93.5(2006):887-895. |
入库方式： OAI收割
来源：大连化学物理研究所
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