CE-LIF coupled with flow cytometry for high-throughput quantitation of fluorophores in single intact cells
文献类型：期刊论文
| 作者 | Xiao, Hua; Li, Xin; Zou, Hanfa; Yang, Ling; Wang, Yulin; Wang, Hailin; Le, X. Chris |
| 刊名 | electrophoresis
 |
| 出版日期 | 2006-09-01 |
| 卷号 | 27期号:17页码:3452-3459 |
| 关键词 | apparent permeability coefficient capillary electrophoresis flow cytometry single cell analysis transport rate |
| ISSN号 | 0173-0835 |
| 产权排序 | 1;1 |
| 通讯作者 | 邹汉法 |
| 英文摘要 | we report a method of coupled ce-lif detection with flow cytometry for high-throughput determination and quantitation of fluorophores in single intact k562/s (ks) cells. the membrane properties of ks cell including fluophore transport rate and apparent permeability coefficient were further quantitatively characterized. the method has advantages for accurate quantitation and unique capacity of high-throughput analysis. the strategy will be useful for the quantitation of fluorophores in the intact cells, such as measurement of multidrug resistance, quantitation of specific protein expression, and quantitative characterization of protein and enzyme functions. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | induced fluorescence detection ; capillary-electrophoresis ; multidrug-resistance ; caco-2 monolayers ; mammalian-cells ; microscopy ; transport ; effluxes ; protein |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000240585900016 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/97219]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Pharmaceut Resource Discovery, Dalian, Peoples R China 3.Univ Alberta, Fac Med, Dept Publ Hlth Sci, Edmonton, AB, Canada |
| 推荐引用方式 GB/T 7714 | Xiao, Hua,Li, Xin,Zou, Hanfa,et al. CE-LIF coupled with flow cytometry for high-throughput quantitation of fluorophores in single intact cells[J]. electrophoresis,2006,27(17):3452-3459. |
| APA | Xiao, Hua.,Li, Xin.,Zou, Hanfa.,Yang, Ling.,Wang, Yulin.,...&Le, X. Chris.(2006).CE-LIF coupled with flow cytometry for high-throughput quantitation of fluorophores in single intact cells.electrophoresis,27(17),3452-3459. |
| MLA | Xiao, Hua,et al."CE-LIF coupled with flow cytometry for high-throughput quantitation of fluorophores in single intact cells".electrophoresis 27.17(2006):3452-3459. |
入库方式： OAI收割
来源：大连化学物理研究所
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