Zirconium phosphonate-modified porous silicon for highly specific capture of phosphopeptides and MALDI-TOF MS analysis
文献类型：期刊论文
| 作者 | Zhou, Houjiang; Xu, Songyun; Ye, Mingliang; Feng, Shun; Pan, Chensong; Jiang, Xiaogang; Li, Xin; Han, Guanghui; Fu, Yu; Zou, Hanfa |
| 刊名 | journal of proteome research
 |
| 出版日期 | 2006-09-01 |
| 卷号 | 5期号:9页码:2431-2437 |
| 关键词 | zirconium phosphonate porous silicon phosphopeptide MALDI-TOF MS |
| ISSN号 | 1535-3893 |
| 产权排序 | 1;1 |
| 通讯作者 | 邹汉法 |
| 英文摘要 | phosphorylation is one of the most important post-translational modifications of proteins, which modulates a wide range of biological functions and activity of proteins. the analysis of phosphopeptides is still one of the most challenging tasks in proteomics research by mass spectrometry. in this study, a novel phosphopeptide enrichment approach based on the strong interaction of zirconium phosphonate (zrp) modified surface with phosphopeptides has been developed. zrp modified porous silicon (zrppsi) wafer was prepared to specifically capture the phosphopeptides from complex peptide mixtures, and then the captured phosphopeptides were analyzed by maldi-tof ms by directly placing the wafer on a maldi target. the phosphopeptide enrichment and maldi analysis were both performed on the zrp-psi wafer which significantly reduced the sample loss and simplified the analytical procedures. the prepared zrp-psi wafer has been successfully applied for the enrichment of phosphopeptides from the tryptic digest of standard phosphoproteins, beta-casein and alpha-casein. the excellent selectivity of this approach was demonstrated by analyzing phosphopeptides in the digest mixture of beta-casein and bovine serum albumin with molar ratio of 1:100. high detection sensitivity has been achieved for the analysis of the phosphopeptides from tryptic digestion of 2 fmol, beta-casein on the zrp-psi surface. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemical research methods |
| 研究领域[WOS] | biochemistry & molecular biology |
| 关键词[WOS] | desorption/ionization-mass-spectrometry ; ion affinity-chromatography ; phosphorylated peptides ; protein-phosphorylation ; phosphoproteome analysis ; selective enrichment ; identification ; platform ; ms/ms ; nanoparticles |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000240200700041 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/97235]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Houjiang,Xu, Songyun,Ye, Mingliang,et al. Zirconium phosphonate-modified porous silicon for highly specific capture of phosphopeptides and MALDI-TOF MS analysis[J]. journal of proteome research,2006,5(9):2431-2437. |
| APA | Zhou, Houjiang.,Xu, Songyun.,Ye, Mingliang.,Feng, Shun.,Pan, Chensong.,...&Zou, Hanfa.(2006).Zirconium phosphonate-modified porous silicon for highly specific capture of phosphopeptides and MALDI-TOF MS analysis.journal of proteome research,5(9),2431-2437. |
| MLA | Zhou, Houjiang,et al."Zirconium phosphonate-modified porous silicon for highly specific capture of phosphopeptides and MALDI-TOF MS analysis".journal of proteome research 5.9(2006):2431-2437. |
入库方式： OAI收割
来源：大连化学物理研究所
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