Screening and analysis of an antineoplastic compound in Rhizoma Chuanxiong by means of in vitro metabolism and HPLC-MS
文献类型：期刊论文
| 作者 | Kong, Liang; Yu, Zhiyuan; Bao, Yongming; Su, Xingye; Zou, Hanfa; Li, Xin |
| 刊名 | analytical and bioanalytical chemistry
 |
| 出版日期 | 2006-09-01 |
| 卷号 | 386期号:2页码:264-274 |
| 关键词 | coniferyl ferulate ferulic acid ethyl ester HPLC-MS rhizoma chuanxiong traditional Chinese medicine |
| ISSN号 | 1618-2642 |
| 产权排序 | 1;1 |
| 通讯作者 | 邹汉法 |
| 英文摘要 | a new screening and analysis method that combines in vitro metabolism with high-performance liquid chromatography-mass spectrometry (hplc-ms) was developed for the screening and analysis of an antineoplastic compound, coniferyl ferulate, which is present in the rhizome of rhizoma chuanxiong. infrared (ir), ultraviolet visible spectroscopy (uv-vis), nuclear magnetic resonance (nmr) and element analysis were used to identify the molecular structure of coniferyl ferulate. the quantitative analysis of coniferyl ferulate in different extracts of rhizoma chuanxiong was carried out, and the metabolism of coniferyl ferulate was investigated by in vitro incubation with rat liver homogenate. the metabolite of coniferyl ferulate, ferulic acid ethyl ester, was identified by hplc-ms, uv-vis and ir. in addition, antineoplastic activities of coniferyl ferulate and ferulic acid ethyl ester were detected by the mtt assay. the observed inhibition rate of coniferyl ferulate on the activity of hela cells was over 80% at 5.4 ng mu l(-1). however, its metabolite, ferulic acid ethyl ester, showed no antineoplastic activity in vitro. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | performance liquid-chromatography ; mass-spectrometry ; ferulic acid ; cnidium-officinale ; angelica-sinensis ; tetramethylpyrazine ; hydroxyphthalides ; pregnenolone ; ingredients ; components |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000240281400010 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/97285]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 2.Dalian Univ Technol, Sch Environm & Biol Sci & Technol, Dalian 116024, Peoples R China |
| 推荐引用方式 GB/T 7714 | Kong, Liang,Yu, Zhiyuan,Bao, Yongming,et al. Screening and analysis of an antineoplastic compound in Rhizoma Chuanxiong by means of in vitro metabolism and HPLC-MS[J]. analytical and bioanalytical chemistry,2006,386(2):264-274. |
| APA | Kong, Liang,Yu, Zhiyuan,Bao, Yongming,Su, Xingye,Zou, Hanfa,&Li, Xin.(2006).Screening and analysis of an antineoplastic compound in Rhizoma Chuanxiong by means of in vitro metabolism and HPLC-MS.analytical and bioanalytical chemistry,386(2),264-274. |
| MLA | Kong, Liang,et al."Screening and analysis of an antineoplastic compound in Rhizoma Chuanxiong by means of in vitro metabolism and HPLC-MS".analytical and bioanalytical chemistry 386.2(2006):264-274. |
入库方式： OAI收割
来源：大连化学物理研究所
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